喹诺酮类和磺胺类抗生素对绿藻生长的影响

Effects of quinolones and sulfonamides on the growth of green algae

喹诺酮类抗生素(氧氟沙星,OFLX)和磺胺类抗生素(磺胺间甲氧嘧啶,SMM;磺胺二甲基嘧啶,SMZ)是目前自然水体中含量较高的两类抗生素,研究其对水环境的生态效益意义重大。采用单因素方法分析绿藻对不同浓度抗生素的敏感性,同时定量分析3种抗生素在单一及联合作用下对绿藻生长的半抑制浓度。结果表明:单一及联合抗生素对淡水绿藻均表现为"低促高抑",抗生素浓度≤5μg/mL促进藻类的生长,>5μg/mL抑制藻类的生长;OFLX、SMM、SMZ对普通小球藻的半抑制浓度分别为:7.96、6.58、8.19μg/mL;对斜生栅藻的半抑制浓度分别为:6.20、7.20、9.89μg/mL;OFLX+SMM对普通小球藻的半抑制浓度为8.28μg/mL,对斜生栅藻的半抑制浓度为5.71μg/mL;OFLX+SMM+SMZ对普通小球藻的半抑制浓度为10.23μg/mL,对斜生栅藻的半抑制浓度为7.40μg/mL。抗生素对淡水绿藻的生长表现为"低促高抑",OFLX和联合抗生素对斜生栅藻敏感,SMM、SMZ对普通小球藻更为敏感。

The quinolone antibiotics（Ofloxacin,OFLX） and sulfa antibiotics（sulfamonometh-oxine,SMM,sulfamethazine,SMZ） are the two types of antibiotics with high levels in natural waters.The ecological benefits to the water environment are significant.The susceptibility of green algae to different concentrations of antibiotics was analyzed by single factor method.At the same time,the semi-inhibitory concentrations of green algae under the single and combined effects of the three antibiotics were quantitatively analyzed.Both single and combined antibiotics showed ＂ low growth and high inhibition＂ for freshwater green algae,antibiotics at the concentration of ≤5μg/mL promoted the growth of algae,when 5μg/mL the growth of algae was inhibited.The semi-inhibitory concentrations of OFLX,SMM,SMZ to C.vulgaris were 7.96 μg/mL,6.58 μg/mL,and 8.19 μg/mL respectively,and the semi-inhibitory concentrations to S.obliquus were 6.20 μg/mL,7.20 μg/mL,9.89μg/mL respectively.The half-inhibitory concentration of OFLX ＋ SMM to C.vulgaris was 8.28μg/mL,and the semi-inhibitory concentration to S.obliquus was 5.71μg/mL.The semi-inhibitory concentration of OFLX＋SMM＋SMZ to C.vulgaris was 10.23 μg/mL,and the semi-inhibitory concentration to S.obliquus was 7.40 μg/mL.Antibiotics showed ＂ low growth and high inhibition ＂ on the growth of freshwater green algae,OFLX and combined antibiotics were sensitive to S.obliquus,and SMM and SMZ were more sensitive to C.vulgaris.