探讨荧光免疫法检测乳腺癌组织中hMAN的可行性

Study on the Feasibility of Fluorescence Immunoassay Method to Detect Human Mammaglobin in Breast Globin

目的用免疫荧光法来检测乳腺不同组织中乳腺珠蛋白的表达,探讨免疫荧光检测乳腺癌特异蛋白的可行性。方法用免疫荧光法检测乳腺癌组织、乳腺正常组织和乳腺纤维瘤组织中乳腺珠蛋白的表达。结果乳腺珠蛋白在乳腺癌组织与乳腺正常组中的表达差异有统计学意义(p=0. 0019,p <0. 01);乳腺珠蛋白在乳腺纤维瘤组和乳腺正常组中的表达(p=0. 071,p> 0. 05)没有统计学意义。结论免疫荧光法可用来检测乳腺癌组织中的乳腺珠蛋白。

Objective： To study the feasibility of fluorescence immunoassay method on detecting breast cancer specific proteins through checking human mammaglobin expression in different mammary gland tissues.Methods： Use immunofluorescence method to detect human mammaglobin expression in breast cancer tissues, normal breast tissue and mammary gland fibroma.Results： Human mammaglobin expression had statistical significant differences between breast cancer tissue and normal breast tissue （p=0.0019,p〈0.01） ,no statistical significance between mammary gland fibroma and normal breast tissue （p=0.071, p〉0.05） .Conclusion： Immunofluorescence method could be used to detect human mammaglobin in breast cancer tissue.