双波长高效液相色谱法同时测定复方黑参口服溶液中5种成分含量

Simultaneous Determination of Five Components in Fufang Heishen Oral Liquid by Dual-wavelength HPLC

目的建立同时测定复方黑参口服溶液中5种成分含量的高效液相色谱法。方法色谱柱为Agilent Hypersil ODS(4. 6 mm×250 mm,5μm),流动相为乙腈(A)-0. 1%磷酸溶液(B),梯度洗脱,柱温:30℃,流速:1.0 mL·min^(-1),检测波长:210,270 nm。结果哈巴苷在7. 12～85. 44 mg·L^(-1)(r=0. 9999);哈巴俄苷在2. 50～30.00 mg·L^(-1)(r=0.9998);肉桂酸在25.3～304.20 mg·L^(-1)(r=0.9997);射干苷在0.73～8.70 mg·L^(-1)(r=0.9997);次野鸢尾黄素在1.20～14.40 mg·L^(-1)(r=0.9998)浓度范围内线性关系良好。平均加样回收率分别为98.8%,102.7%,98.8%,99.3%,99.9%,RSD分别为1.23%,2.89%,2.60%,1.44%,2.84%(n=6)。结论所建立的方法简便准确,可用于复方黑参口服液中的哈巴苷、哈巴俄苷、肉桂酸、射干苷、次野鸢尾黄素的含量测定。

Objective To establish a method that could detect 5 components of Fufang Heishen oral liquid simultaneously.Methods The component was performed by high performance liquid chromatography （HPLC） equipped with Agilent Hypersil ODS （4.6 mm×250 mm,5 μm）.The mobile phase consisted of acetonitrile-0.1% Phosphoric acid with gradient elution.The flow rate was 1.0 mL·min -1 with the 210 nm and 270 nm detection wavelength,20 μL injection volume and 30 ℃ column temperature.Results A good linear relationship was observed with the range of 7.12-85.44 mg·L -1 for Harpagide（ r = 0.999 9）,2.50-30.00 mg·L -1 for Harpagoside（ r =0.999 8）,25.35-304.20 mg·L -1 for Cinnamic acid（ r =0.999 7）,0.73-8.70 mg·L -1 for Tectoridin（ r =0.999 7）and 1.20-14.40 mg·L -1 for Irisflorentin（ r =0.999 8）.The average recovery of each detected component of Fufang Heishen Oral Liquid was 98.8%,102.7%,98.8%,99.3%,99.9% the RSD were 1.23%,2.89%, 2.60% ,1.44%,2.84%（ n =6）.Conclusion The method is simple,rapid and accurate and can be used to detect the content of Harpagide,Harpagoside,Cinnamic acid,Tectoridin and Irisflorentin of Fufang Heishen Oral Liquid.