基于多指标成分的舒胃合剂质量评价

Quality Evaluation of Shuwei Mixture Based on Multi-index Constituents

目的通过测定芥子碱硫氰酸盐、厚朴酚、和厚朴酚、柚皮苷、橙皮苷和新橙皮苷的含量对舒胃合剂进行质量评价。方法采用反相高效液相色谱法(RP-HPLC),Agilent ZORBAX Eclispse SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 mL·min^(-1),检测波长分别为326 nm(芥子碱硫氰酸盐),294 nm(厚朴酚、和厚朴酚),283 nm(柚皮苷、橙皮苷、新橙皮苷),柱温30℃。结果芥子碱硫氰酸盐、厚朴酚、和厚朴酚、柚皮苷、橙皮苷和新橙皮苷分别在0.049 6～1.24,0.048 2～1.205,0.060 5～1.512 5,0.187 2～4.68,0.131 6～3.29,0.197～4.925μg线性关系良好; 6种成分平均回收率分别为100.66%,99.86%,101.37%,102.41%,99.01%,102.05%,RSD分别为0.82%,1.89%,2.56%,0.74%,1.54%,0.99%(n=6)。结论该方法简便,准确可靠,重复性好,专属性好,分离效果好,适用于舒胃合剂的质量评价。

Objective The quality evaluation of the Shuwei mixture was determined by the content of the six components of sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin.Methods RP-HPLC method was used.The separation was performed on a Agilent ZORBAX Eclispse SB-C 18 column （4.6 mm×250 mm,5 mm）,the mobile phase consisted of acetonitrile（A）-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 mL·min -1 .The detection wavelength was 326 nm（sinapine cyanide sulfonate）,294 nm（magnolol, honokiol）and 283 nm（naringin, neohesperidin）.The column temperature was kept at 30 ℃.Results The sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin all had good linear relationship in the ranges of 0.049 6-1.24,0.048 2-1.205,0.060 5- 1.512 5 ,0.187 2-4.68,0.131 6-3.29,0.197-4.925 μg.The average recoveries were 100.66%, 99.86%, 101.37%, 102.41%, 99.01% , 102.05%, respectively, RSD were 0.82%, 1.89%, 2.56 %, 0.74%, 1.54%, 0.99%, respectively.Conclusion The method is simple,accurate,reproducible and nice to the separation,and can be used for the quality evaluation of Shuwei mixture.