抑制性分子LAIR-1在异种移植物杀伤机制中的作用

The role of inhibitory molecule LAIR-1 in the killing mechanism of xenografts

目的探讨NK细胞表面抑制性受体白细胞相关免疫球蛋白样受体-1(eukocyte-associated immunoglobulin like receptor-1,LAIR-1)在异种移植免疫排斥反应中的作用。方法采用免疫组织化学染色检测异种移植术后供肝组织的免疫细胞浸润情况,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测受体血浆中可溶性LAIR-1(s LAIR-1)的表达,转录组测序检测受体免疫细胞表面膜型LAIR-1(m LAIR-1)的表达。结果异种移植术后供肝组织受到淋巴细胞浸润,受体淋巴细胞表面mLAIR-1和s LAIR-1呈动态平衡状态。结论受体免疫细胞表面mLAIR-1和血浆中sLAIR-1的表达水平处于动态平衡的状态可能与受体发生免疫排斥反应相关,LAIR-1有望成为移植术后诱导免疫耐受的分子。

Objective To detect the functional significance of surface inhibitory receptor leukocyte- associated immunoglobulin like receptor-1 （LAIR-1） of NK cells in xenogeneic immune rejection. Methods The infiltration of immune cells was detected by the iininunohistocheinical staining in the donor liver tissue after xenotransplantation. The soluble LAIR-1 （sLAIR-1） in the recipient plasma and membrane LAIR-1 （mLAIR-1） in recipient immune cells were detected by the enzyme linked immunosorbent assay （ELISA）and RNA sequencing, respectively. Results In tissue level, the donor liver was infiltrated of lymphoeytes. In molecular level, the sLAIR-1 and mLAIR-1 were in equilibrium. Conclusion The mLAIR-lof NK cell and sLAIR-1 in plasma were in balance and related to xenogeneie immune rejection. Taken all together these data suggest that the LAIIR-1 represents an important target in inducing immunological tolerance.