TGF-β1基因多态性与头颈部肿瘤遗传易感性关系的Meta分析

Relationship betw een TGF-β1 polym orphism and genetic susceptibility in head and neck cancer:A Meta analysis

目的:综合分析TGF-β1基因多态性与头颈部肿瘤遗传易感性之间的关系。方法:按照统一的检索策略,在Pubmed、Embase、万方、CNKI等中英文数据库中全面检索,收集2017年12月31日之前发表的关于TGF-β1基因多态性与头颈部肿瘤易感性关系的相关文献,然后按照纳入及排除标准筛选文献,提取数据和评价质量后应用Stata12. 0软件进行数据分析。结果:共纳入10个病例对照研究,分别对包含869T/C位点的8篇,509C/T位点的5篇,915G/C位点的3篇文章进行统计分析。在869 T/C研究中我们观察到:病例组1 607例,对照组1 981例,总体分析显示其与头颈部肿瘤之间有关联,OR=1. 181,95%CI:1. 035～1. 348,P=0. 014。对不同病种、不同人种、样本量大小进行亚组分析显示,869T/C各基因位点与鼻咽癌和口腔癌这两种病种无关联,与亚裔人种有关联,OR=1. 431,95%CI:1. 191～1. 720,P=0. 000。该位点多态性同时与样本量大于100有关联性,OR=1. 230,95%CI:1. 078～1. 404,P=0. 002。在509C/T的5篇研究中:病例组1 355例,对照组1 579例,我们仅分析了鼻咽癌相关的4项研究,很遗憾我们并没有发现该位点与鼻咽癌之间有关联,OR=1. 090,95%CI:0. 865～1. 374,P=0. 464。在915G/C位点的3篇研究中我们共收集了病例组340例,对照组368例,均为口腔癌,统计结果显示该位点与口腔癌之间有关联,OR=2. 815,95%CI:1. 581～5. 012,P=0. 000。在等位基因和显性基因中显示出有统计学差异(C vs G:OR=3. 800,95%CI:1. 125～12. 843,P=0. 032; GC+CC vs GG:OR=5. 113,95%CI:1. 224～21. 367,P=0. 025);其余基因型未显示统计学差异。同时分别对三种基因位点的相关研究进行敏感性分析显示结果稳定,Begger和Egger检验均提示无发表偏倚。结论:Meta分析显示869T/C基因与头颈部肿瘤的遗传易感性相关,在亚组分析中与亚洲人种和样本量大于100有关联性; 915G/C等位基因C vs G,显性基因GC+CC vs GG与口腔癌发生有关,但是509C/T基因与头颈部肿瘤遗传易感性无关。

Objective To evaluate the relationship between TGF-β1 gene polymorphism and genetic predisposition of head and neck cancer.Methods： According to a unified search strategy,a comprehensive search was carried out in various Chinese and English databases to collect relevant literature had been published before December 31,2017 on the relationship between TGF-β1 gene polymorphism and head and neck cancer,then screening literature according to inclusion and exclusion criteria,extracting the data and evaluating the quality of data,Stata12.0 software was used for data analysis.Results： A total of 10 case-control studies were conducted.8 articles about 869T/C genes,5 articles with 509C/T genes and 3 articles with 915G/C genes were analyzed statistically.In the 869T/C study,we observed 1 607 patients in the case group and 1 981 in the control group.The overall analysis showed a correlation with head and neck cancers,OR=1.181,95%CI：1.035~1.348, P =0.014.We also analyzed on different subgroup including cancer type,sampel size and ethnicities,it did not show that 869T/C gene associated with nasopharyngeal carcinoma and oral both diseases,it associated with both the Asian （OR=1.431,95%CI：1.191~1.720, P =0.000） and sample size that more than 100 （OR=1.230,95%CI：1.078~1.404, P =0.002）.In 5 studies at 509C/T,1 355 cases and 1 579 controls we analyzed only four studies related to nasopharyngeal cancer,and we failed to verify the link between the 509C/T and nasopharyngeal carcinoma,OR=1.090,95%CI：0.865~1.374, P =0.464.In three studies about the 915G/C,we collected a total of 340 cases in the case group and 368 in the control group,the statistical results showed that the gene was associated with head and neck cancer,OR=2.815,95%CI：1.581~5.012, P =0.000.There was a statistically significant difference between alleles and dominant genes （C vs G：OR=3.800,95%CI：1.125~12.843, P =0.032, GC＋CC vs GG：OR=5.113,95%CI： 1.224~21.367, P =0.025）.The recessive and homozygous genes showed no difference.At the same time,sensitivity analysis of the three genes related studies showed that the results were stable,Begger and Egger test were showed no publication bias.Conclusion： We concluded that 869T/C associated with the genetic predisposition of head and neck cancer.In subgroup analysis,there was a correlation between Asian population and sample size more than 100.The C vs G allele and the dominant gene GC＋CC vs GG of 915G/C were associated with the genetic predisposition of head and neck cancer.However,Meta-analysis failed to show a statistical association between TGF-β1-509C/T and HNC.