3种培养基对微藻FACHB-1067生长及产油性状的影响研究

Effects of Three Different Mediums on the Growth Performance and Oil Production of Microalgae FACHB-1067

为评价不同培养基对微藻FACHB-1067生长性状及油脂积累的影响,采用BG11、BG11-N、SE 3种不同培养基,在温度20℃、光照2 084 lx、光暗周期16 h∶16 h的条件下对微藻FACHB-1067进行培养;考查微藻FACHB-1067在不同培养基中的生物积累量、比生长速率、油脂含量及油脂产率等指标,并采用统计学方法进行比较分析。结果表明,微藻FACHB-1067在SE培养基中的比生长速率最快,为1.052/d;其在3种培养基中的生物积累量由大到小依次为:SE(2.532 g/L)>BG11(2.032 g/L)>BG11-N(1.916 g/L);其在BG11培养基中的油脂含量和油脂产率最高,分别为28.64%和1.628 mg/(L·d),显著高于其在BG11-N和SE培养基中的油脂含量及油脂产率(P<0.05)。综上表明,SE培养基在比生长速率和生物积累量上的表现较理想,而BG11培养基在油脂含量和油脂产率上的表现较理想。

To evaluate the effects of different mediums on growth characteristics and oil accumulation of microalgae FACHB-1067, three different mediums, including BG11, BG11-N and SE, were used to culture microalgae FACHB-1067 under the conditions of 20 ℃, 2 084 lx light and 16 h∶16 h light-dark cycle. The bioaccumulation, specific growth rate, oil content and oil yield of microalgae FACHB-1067 in different mediums were investigated and compared by statistical method. The results showed that the specific growth rate of microalgae FACHB-1067 in SE medium was the fastest, which was 1.052/d; the ranking of the biomass accumulation in three mediums was SE （2.532 g/L） 〉BG11 （2.032 g/L） 〉BG11-N （1.916 g/L）; the oil content and yield in BG11 medium were 28.64% and 1.628 mg/（L·d）, respectively, which were significantly higher than those in BG11-N and SE medium （P〈0.05）. In summary, SE medium showed better performance in specific growth rate and biomass accumulation, while BG11 medium showed better performance in oil content and oil yield.