四唑染色法快速测定菊芋种子生活力的研究

Rapid Determination of Seed Viability of Helianthus tuberosus by Using Tetrazole Method

为了研究不同四唑(TTC)溶液浓度、不同染色温度对菊芋种子生活力测定的影响。根据四唑染色法测定种子生活力的原理,对菊芋种子生活力的最佳条件进行了测定。结果表明,当菊芋种子在25℃蒸馏水中浸种,待种子完全吸胀后,于35℃黑暗条件下、TTC浓度0.5%,染色7 h,染色效果最好,可以很好地检测菊芋种子活力水平,估计种子潜在发芽能力,为菊芋种子生活力的快速测定提供参考。

In order to investigation the effect of different concentrations of tetrazole solution and different dyeing temperature on the determination of seed viability of Helianthus tuberosus, the optimum conditions of seed viability of Helianthus tuberosus determination were screened according to the principle of tetrazole dyeing method （TTC）. The results showed that the best dyeing effect was obtained under the following conditions： after completely soaked in the distilled water at 25 %, the seeds of Helianthus tuberosus were dyed using TTC with concentration of 0.5% for 7 hours under dark condition of 35 %. The seeds viability can be well determined and their potential germination ability can be well estimated using the above method. This study provides references for the rapid determination of seed viability of Helianthus tuberosus.