PBX3在宫颈癌中的表达及与临床病理参数和预后关系

The expression of PBX3 in cervical carcinoma tissue and its relationships with clinicopathological features and prognosis

目的:探究宫颈癌组织中前B细胞白血病同源盒基因3(PBX3)的表达,以及与临床病理特征及预后的关系。方法:收集本院宫颈癌以及癌旁组织82例进行研究,Western blot法、免疫组化法检测标本组织中PBX3蛋白表达,分析二者与临床病理特征及预后的关系。对影响患者生存的临床因素进行COX回归分析,所有患者均进行30个月随访,统计患者生存率,采用Kaplan-Meier分析患者生存情况,Log-Rank法检测组间生存差异。结果:与癌旁组织相比,宫颈癌组织中PBX3蛋白、蛋白阳性表达率均升高(P<0.05)。PBX3表达与年龄、病理类型、肿瘤浸润深度无关(P>0.05),与肿瘤分期、肿瘤分化程度、肿瘤直径、浸润程度淋巴结转移有关(P<0.05)。30个月随访,24人死亡,死亡率为29.3%。COX多因素分析显示,肿瘤分期、肿瘤分化程度、肿瘤直径、淋巴结转移、PBX3为影响患者预后的独立因素。PBX3阳性表达组58.1%死亡,阴性表达组23.5%死亡,两组患者Kaplan-Meier生存曲线存在差异(P<0.05)。结论:PBX3在宫颈癌组织中表达上调,与肿瘤分期、分化程度、淋巴结转移等有关,可能为宫颈癌患者预后的生物学指标。

Objective： To investigate the expression of pre leukemia transcription factor 3 （PBX3） in cervical carcinoma tissue, and to analyze its relationships with clinicopathological features and prognosis. Methods： The cervical carcinoma and para cancerous tissues of 82 women were collected. The expression of PBX3 protein in these tissues was detected by Western blot and immunohistochemistry. The relationships of different tissues with the clinicopathological features and prognosis were analyzed. COX regression was performed for analysis the clinical factors which might be affected the survival of patients. All women were followed up for 30 months, and their survival rates were analyzed by Kaplan Meier, and the difference of survival of women between the two groups was detected by Log Rank method. Results： Compared with those in para cancerous tissue, the positive expression rate of PBX3 protein in cervical cancer tissues was significant higher （ P 〈 0.05）. The expression of PBX3 protein was not related to age, pathological type or depth of tumor invasion （ P 〉0.05）, but which was related to tumor staging, tumor differentiation, tumor diameter, and infiltration degree lymph node metastasis （ P 〈0.05）. In the 3 year follow up, there was 30 people died, and the mortality rate was 36.59%. COX multivariate analysis showed that tumor staging, tumor differentiation, tumor diameter, lymph node metastasis and PBX3 were independent factors affected the prognosis of the patients. 18 cases died in PBX3 positive expression group, and 12 cases died in PBX3 negative expression group. Kaplan Meier survival curve showed that there was significant difference in Kaplan Meier survival curve between the two groups （ P 〈0.05）. Conclusion： The up regulated expression of PBX3 in cervical carcinoma is related to the stage of tumor, degree of differentiation, lymph node metastasis and so on, so it may be a biological indicator of the prognosis of cervical cancer patients.