木贼麻黄多糖的提取及体外抗氧化活性研究

Research of Polysaccharides Extraction from Ephedra Equisetina Bunge. and Its Antioxidant Activity in Vitro

目的优化木贼麻黄多糖的提取工艺,初步研究木贼麻黄多糖的体外抗氧化活性。方法采用热水浸提法提取木贼麻黄多糖,利用单因素试验结合响应面法对提取条件进行优化,并研究木贼麻黄多糖的体外抗氧化活性。结果木贼麻黄多糖的最佳提取条件为:提取时间2.5 h,提取温度73℃,液料比25∶1,在此条件下多糖提取率为(5.57±0.072)%。木贼麻黄多糖对DPPH自由基和羟基自由基具有较强的清除能力,IC_(50)分别为703.9μg·mL^(-1)和2 135.1μg·mL^(-1);对超氧阴离子自由基具有一定的清除能力,对Fe^(3+)有一定的还原能力。结论该提取工艺能较好地提取木贼麻黄多糖,所得木贼麻黄多糖具有一定的体外抗氧化能力,但活性低于同浓度的维生素C。

OBJECTIVE To optimize the extraction condition of the polysaccharides from Ephedra equisetina Bunge. and study its antioxidant activity in vitro. METHODS The polysaccharides were extracted from Ephedra equisetina Bunge. by hot water extraction. The single factor tests and response surface methodology were used to optimize the extraction condition. The antioxidant activity in vitro was evaluated by artificial free radicals in detail. RESULTS The optimum extraction condition was： extraction time 2.5 h, extraction temperature 73 ℃, ratio of liquid to material 25∶1. Under the optimum condition, the yield was（5.57±0.072）%. The antioxidant activity in vitro showed that the polysaccharide from Ephedra equisetina Bunge. had a better scavenging effect on DPPH radical and hydroxyl radical with IC（50） of 703.9 μg·mL（-1） and 2 135.1 μg·mL（-1） respectively, and had a lower scavenging effect on superoxide anion radical and weak reductive effect on Fe（3＋）. CONCLUSION The extraction condition is suitable to extract the polysaccharides from Ephedra equisetina Bunge. and the polysaccharide has certain antioxidant capacity in vitro but less than vitamin C.