雷公藤多苷片对高脂小鼠血脂的影响

Study on the Effect of Tripterygium Glycosides on Hyperlipidemia Mice

目的研究雷公藤多苷片对高脂小鼠血脂的影响。方法采用高血脂小鼠模型,通过小鼠尾静脉注射不同剂量(0.5,1.0,1.5 mg·kg^(-1)·d^(-1))雷公藤多苷,测定不同时间段小鼠体质量和血清总胆固醇(total cholesterol,TC)、甘油三酯(total triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)的含量及卵磷脂胆固醇酰基转移酶(lecithin cholesterol acyltransferase,LCAT)、肝脂酶(hepatic lipase,HL)和脂蛋白脂酶(lipoprotein lipase,LPL)的活性,研究雷公藤多苷对小鼠体质量、血脂代谢、动脉粥样硬化指数、肝脏LCAT水平等的影响。结果给予高脂饲料后小鼠体质量和血脂显著升高(P<0.01),动脉粥样硬化指数显著升高(P<0.01),抗动脉粥样硬化指数显著下降(P<0.01)。注射雷公藤多苷能显著降低高血脂模型小鼠血清TC、TG和LDL-C,同时显著降低高脂饮食所导致的动脉粥样硬化指数升高。此外,雷公藤多苷片能显著提高血清LCAT水平和HL、LPL的活性。结论雷公藤多苷片具有一定的降血脂功能,并能有效降低动脉粥样硬化的发生几率。

OBJECTIVE To investigate the effect of tripterygium glycosides on hyperlipidemia mice in vivo. METHODS The hyperlipidemia mice model was established by feeding with high-fat diet. Low-, middle-and high-dose tripterygium glycosides groups were injected intravenously at dosages of 0.5, 1.0, 1.5 mg·kg（-1）·d（-1）, respectively. The changes in body weight, serum total cholesterol（TC）, total triglyceride（TG）, high density lipoprotein cholesterol（HDL-C）, low density lipoprotein cholesterol（LDL-C）, the activity of lecithin cholesterol acyltransferase（LCAT）, hepatic lipase（HL） and lipoprotein lipase（LPL） were measured. At the same time, the atherosclerosis index（AI） and anti-atherogenic index（AAI） were measured to explore the effect of tripterygium glycosides on atherosclerosis. RESULTS Body weight, serum lipids and AI were significantly increased after feeding with high-fat diet（P0.01）, while AAI was significantly decreased（P0.01）. Tripterygium glycosides could significantly reduce serum TC, TG and LDL-C, and significantly inhibit AI induced by high-fat diet in hyperlipidemic mice. In addition. the level of LCAT and activity of HL and LPL were significantly increased after treat with tripterygium glycosides. CONCLUSION Tripterygium glycosides has hypolipidemic effect and it can decrease the rate of atherosclerosis.