牡荆素在大鼠体内的代谢研究

Metabolic Research of Vitexin in Rats

目的研究黄酮碳苷牡荆素在大鼠体内的代谢产物,并推测其代谢途径。方法 SD大鼠灌胃给予5 mg·kg^(-1)牡荆素,收集0~3 h,3~6 h,6~12 h的尿液,采用UPLC-Q-TOF检测尿样中代谢产物。结果采用Metabolynx XS代谢物分析软件,根据质谱碎片信息对代谢物进行结构鉴定,最终得到3个代谢产物。结论牡荆素在大鼠尿液中检测得到1个一相代谢产物,2个二相代谢产物,推测牡荆素在大鼠体内主要发生氧化、甲基化和葡萄糖醛酸结合反应,其中葡萄糖醛酸化反应是较强的代谢种类。

OBJECTIVE To investigate the metabolites of flavonoid vitexin in rats, and to identify its metabolic pathways. METHODS The SD rats were intragastric administration vitexin with a dose of 5 mg·kg（-1）. The urine were collected at 0-3 h,3-6 h,6-12 h after drug administration. UPLC-Q-TOF was applied to separate and analyze vitexin and its metabolites in rat urine. RESULTS A total of 3 vitexin metabolites were detected and identified by Metabolynx XS software, which structural identification was based on mass fragment information. CONCLUSION The results show that vitexin is detected in rat urine as a phase I and two phase II metabolisms. It is speculated that oxidation, methylation and gluconic acid conjugation reactions are the main metabolic pathways of vitexin in rat in vivo, and gluconic acid conjugation is a strong metabolic species.