合生元对慢性功能性便秘患者肠道菌群的影响

Effect of synbiotics on the intestinal microbiota in patients with chronic functional constipation

目的 分析合生元在治疗慢性功能性便秘过程中对肠道菌群的改善情况.方法 根据纳入和排除标准,选取6例慢性功能性便秘患者,留取新鲜粪便,在经过合生元持续治疗1个月后,再次留取粪便.同时选取6名健康志愿者的粪便作为对照组,粪便样本送至实验室并存放于-80℃冰箱.采用宏基因组测序技术对肠道菌群进行检测.结果 经合生元治疗4周后,患者的肠道菌群在种水平发生了改变.大肠埃希菌（Escherichia_ coli）、梭菌类（Clostridium_ sp._SS2/1、Clostridium_ sp._7_3_ 54FAA）在便秘人群中富集,经过治疗后丰度下降.口乳杆菌（Lactobacillus_ oris）和动物双歧杆菌（Bifidobacterium_ animalis）在健康人群中富集,经过治疗后升高.韦荣氏球菌（Veillonella_ parvula、Veillonella_ sp._6_1_27、VeiUoneUa_ sp._3_1_44）在健康人群中富集,经过治疗后含量下降.LEfSe分析发现狄氏副拟杆菌（parabacteroidesdistasonis）、阴沟肠杆菌（Escherichia_ coli、enterobacter-cloacae）菌种存在特征性差异.结论 合生元的使用能够改善患者的肠道菌群结构,其通过调节患者的肠道菌群结构,进一步发挥治疗效果,可成为新的临床治疗策略.

Objective To investigate the beneficial role of synbiotics in the intestinal microbiota of patients with chronic functional constipation （CFC）.Methods According to the inclusion and exclusion criteria,6 patients with CFC were enrolled with their fresh fecal samples collected,after a continuous treatment of one month their fresh fecal samples collected again.Meanwhile,6 healthy volunteers were enrolled as the control group with their fresh fecal samples collected.All samples were transported with ice and stored in -80 ℃ refrigerator,and were analyzed by metagenomics sequencing.Results After 4 weeks of symbiotic treatment,the intestinal microbiota had changed in species in patients with CFC.Bacteria of Escherichia_ coli,Clostridium_ sp._ SS2/1 and Clostridium_ sp._ 7_ 3_ 54FAA,which were rich in the people with constipation,decreased in abundance after the treatment.Bacteria of Lactobacillus_ oris and Bifidobacterium _ animalis,which were rich in the healthy people,increased in content after the treatment.Bacteria of Veillonella_ parvula,Veillonella_ sp._ 6_ 1_ 27,Veillonella_ sp._ 3 _ 1_ 44 which were rich in the healthy people,decreased in content after the treatment.LEfSe analysis showed that Parabacteroides distaso nis,Escherichia_ coli and Enterobacter-cloacae were the specific species of the three groups respectively.Conclusion Synbiotics can change the intestinal microbiota showing therapeutic effect,thus can be used as a novel clinical treatment method.