沉默CXCR4基因对胃癌细胞增殖和侵袭能力的影响

Effect of silencing CXCR4 gene on proliferation and invasion of gastric cancer cells

目的探讨沉默CXC型趋化因子受体4(CXCR4)基因对胃癌细胞增殖、侵袭能力的影响。方法培养人胃癌细胞株MKN45,将细胞分为三组:空白组不转染质粒,对照组转染含无义质粒的腺病毒载体,观察组转染含靶向CXCR4-siRNA的腺病毒载体。RT-PCR检测CXCR4mRNA的表达,MTT法检测胃癌细胞增殖情况,Transwell小室检测细胞侵袭能力。结果与观察组相比,空白组和对照组CXCR4 mRNA表达较高(P<0.05)。观察组细胞增殖较空白组和对照组减少(P<0.05)。观察组细胞侵袭数少于对照组和空白组(P<0.05)。结论沉默CXCR4基因能够抑制胃癌细胞的增殖及侵袭能力,CXCR4可能与胃癌的发生、发展有关。

Objective To investigate the effect of silencing CXC chemokine receptor 4（CXCR4）gene on the proliferation and invasion of gastric cancer cells.Methods Gastric cancer cell line MKN45 was cultured and then divided into three groups.The cells of group A were transfected with adenovirus vector carried with CXCR4-siRNA（group A）and those of group B were transfected with nonsense plasmid（group B）.Group C was taken as the blank control.The mRNA expression of CXCR4 was detected by RT-PCR,the cell proliferation was messured by MTT assay and the ability of invasion was detected by Transwell chamber.Results The mRNA expression of CXCR4 was lower in group A than that in groups of B and C（P〈0.05）.The cell proliferation and number of invasion were lower in group A than those in groups of B and C（P〈0.05）.Conclusion Silencing CXCR4 gene can effectively inhibit the proliferation and invasion of gastric cancer cells,which may be related to the occurrence and development of gastric cancer.