西藏不同地区牦牛新孢子虫病血清流行病学调查及防控措施

Sero-epidemiological investigation and prevention and control strategy of neosporiasis in yak in different regions of Tibet

为了调查西藏不同地区牦牛新孢子虫病的流行情况,试验采用酶联免疫吸附法（ELISA）对西藏四个地市（拉萨市、林芝市、那曲市、昌都市）牦牛进行血清流行病学调查,并用SPSS 21.0软件对不同地区的抗体阳性率进行显著性分析。结果表明：牦牛新孢子虫病抗体总阳性率为14.95%（68/455）。其中昌都市阳性率最高,为29.12%（53/182）;其次是林芝市,为16.48%（15/91）;拉萨市和那曲市未检测到阳性样品。从不同地区来看,西藏牦牛新孢子虫病血清抗体阳性率差异显著（P〈0.05）。说明新孢子虫病在西藏不同地区有不同程度的流行,需要引起相关部门的关注。

In order to investigate the prevalence of yak neosporiasis in different regions of Tibet,enzyme-linked immunosorbent assay（ ELISA）was used to carry out sero-epidemiological investigation in yak in four regions of Tibet（ Lhasa,Nyingchi,Nagqu,Qamdo）. SPSS 21. 0 software was used to carry out significance analysis on antibody positive rates in different regions. The results showed that the total antibody positive rate of yak neosporiasis was 14. 95%（ 68/455）. The highest positive rate appeared in Qamdo,being 29. 12%（ 53/182）,followed by Nyingchi with positive rate of 16. 48%（ 15/91）,and no positive samples were detected in Lhasa and Nagqu. Significant difference（ P 0. 05） exsisted in the positive rates of serum antibody between different regions of Tibet. The results suggested that different degrees of the prevalence of neosporiasis appeared in different regions of Tibet,which needed to attract the tention of relevant departments.