小鼠原位肝移植模型的建立与难点解析

Establishment and troubleshooting of orthotopic mouse liver transplantation model

目的建立小鼠原位肝移植模型，并进行难点解析，为器官移植病理和免疫学研究提供实验技术支持。方法选择10-12周龄雄性C57BL／6小鼠为同种移植物供体，雄性C3H小鼠为同种移植物受体。原位肝移植模型建立主要包括三个阶段：供体肝脏获取、供体肝脏修整和供肝移植入受体。供体处理时间平均为（40±8．8）min，供肝处理时间平均为（23±4．7）min，受体手术时间平均为（75±9．6）min。结果完成70对小鼠原位肝移植，主要用于练习显微外科血管和胆道的吻合技术。正式实验同系间移植完成220对，成功199对，成功率为90．4％。结论娴熟、高质量的显微外科技术是小鼠原位肝移植成功的保证。

Objective To construct the orthotopic mouse liver transplantation model and cover troubleshooting, in order to provide experimental techniques support for organ transplantation pathology and immunology studies. Methods Male C57BL/6 mice, 10-12 weeks, were selected as the allograh donors. Male C3H mice with same age were selected as the allograft recipients. The orthotopic mouse liver transplantation model consisted of 3 stages, including harvesting the donor liver, back-table preparation of the liver graft and transplantation of the donor liver into the recipient. The average time for harvesting the donor livers was （40 ± 8.8） min, （23 ±4. 7） min for preparing the donor livers and （75 ±9. 6） min for transplanting the donor livers into the recipient. Results Seventy pairs of mice were used for the preliminary experiments. For the formal experiments, the allograft transplantation was established on 220 pairs with 90. 4% successful rate. Conclusion It is the skillful and high-quality microsurgical technique that is the guarantee of establishing the orthotopic mouse liver transplantation model successfully.