淫羊藿总黄酮胶囊中黄酮类成分含量测定及抗骨质疏松活性研究

Determination of flavonoids constituents in epimedium total flavone capsule and research on its anti-osteoporosis activity

采用快速液相色谱法（RRLC）对淫羊藿总黄酮胶囊进行含量测定,建立了淫羊藿新苷A、朝霍定A1、朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、宝藿苷Ⅱ、淫羊藿次苷Ⅰ、箭藿苷B、鼠李糖基淫羊藿次苷Ⅱ、宝藿苷Ⅰ等11个黄酮类成分的含量测定方法。同时,通过检测碱性磷酸酶（AKP）含量,考察了11个化合物诱导MC3T3-E1细胞增殖分化的效果,结果显示宝藿苷Ⅱ活性最好,宝藿苷Ⅱ和淫羊藿次苷Ⅰ活性都大于淫羊藿苷。该研究建立了黄酮苷类成分的含量测定方法,并比较了每个单体抗骨质疏松的效果,为淫羊藿总黄酮胶囊药效物质基础和作用机制研究提供了技术支持。

To develop a rapid resolution liquid chromatography（RRLC） method for the simultaneous determination of epimedoside A,epimedin A1,epimedin A,epimedin B,epimedin C,icariin,baohuosideⅡ,icarisideⅠ,sagittatoside B,2″-O-rhamnosyl icarisideⅡ,and baohuosideⅠin epimedium total flavone capsule. At the same time,the effects of the above 11 compounds on osteogenic differentiation of MC3 T3-E1 cells were investigated by detecting the content of alkaline phosphatase（AKP）. The results showed that baohuoside Ⅱ had the highest activities,and both the activities of baohuoside Ⅱ and icariside Ⅰ were stronger than those of icariin.In this study,the content determination method of flavonoid glycosides was established,and the anti-osteoporosis effect of monomers was compared,providing technical support for the study of the pharmacodynamic and mechanism of Epimedium total flavone capsule.