决明子炮制过程化学研究

Chemical study on Cassiae Semen processing procedure

决明子炮制后,出现苷类成分含量显著降低,苷元类成分含量明显升高的现象,可能与其所含糖苷酶或炮制过程受热有关,为探明原因,采用高效液相色谱,研究决明子炮制过程中这2种因素对其主要化学成分含量的影响。结果显示,决明子中含有糖苷酶,在温度和水分适宜条件下可快速将决明子中的苷类成分水解为苷元。但在炮制过程中,其糖苷酶并未发挥作用。炮制过程中真正引起决明子中苷含量降低,苷元含量升高的原因是苷在高温条件下苷键断裂分解产生对应的苷元。该实验为进一步揭示决明子的炮制机制提供依据。

References and our previous experiment showed that the contents of glycosides were significantly decreased,while the contents of aglycones were significantly increased after processing of Cassiae Semen. It may be related to its glycosidases or the heating process. In order to investigate the reasons,high performance liquid chromatographic（HPLC） was used to study the effects of these two factors on contents of Cassiae Semen＇s main chemical components in processing. The results showed that glycoside hydrolases was present in Cassiae Semen and could rapidly hydrolyze glycosides from Cassiae Semen into aglycones in suitable temperature with sufficient water. However,it didn＇t show effect on contents change of main constituents in the procedure of Cassiae Semen processing. The reason for content decrease of glycosides and content increase of aglycones in processed Cassiae Semen was glycoside bond cracking to produce corresponding aglycone at high temperature. This study further provides basis for further revealing of the processing mechanism of Cassiae Semen.