槲皮素对人成骨肉瘤MG-63细胞体外增殖与凋亡的影响

Effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells

目的:观察槲皮素对人成骨肉瘤MG-63细胞增殖和凋亡的影响。方法:不同浓度的槲皮素作用于体外培养的MG-63细胞,四甲基偶氮唑盐(MTT)法检测槲皮素对MG-63细胞增殖的抑制能力,流式细胞术(FCM)检测槲皮素对MG-63细胞凋亡的影响。结果:槲皮素可显著抑制MG-63细胞增殖,具有明显剂量和时间依赖性;槲皮素可明显诱导MG-63细胞凋亡。结论:槲皮素能够抑制MG-63细胞增殖,诱导MG-63细胞凋亡。

Objective： To investigate the effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells. Methods： Different concentrations of quercetin were used for in vitro culture of MG-63 cells. MTT assay was used to assess the inhibitory effect of quercetin on the proliferation of MG-63 cells,and flow cytometry was used to evaluate the effect of quercetin on the apoptosis of MG-63 cells. Results： Quercetin significantly inhibited the proliferation of MG-63 cells in a dose-and time-dependent manner. It a lso induced the apoptosis of MG-63 cells. Conclusion： Quercetin can inhibit the proliferation of MG-63 cells and induce the apoptosis of MG-63 cells.