海岛棉GbVIN1基因的克隆与表达分析

Cloning and Expression Analysis of GbVIN1 Gene in Gossypium barbadense L.

根据前期棉纤维发育转录组、表达谱数据分析比较通过PCR技术从海岛棉‘新海21号’中克隆了一个同源基因,命名为GbVIN1,该基因具有一个1 938 bp的开放阅读框,编码645个氨基酸;多序列比对分析表明该蛋白的保守性较高,具有GH32家族保守的-NDPNG-和-WECVD-基序;进化树分析表明,GbVIN1基因与GhVIN1基因处于同一进化树分支。实时荧光定量PCR分析表明,GbVIN1基因在棉纤维发育不同时期中都有表达,且在5 DPA、10 DPA的纤维中表达量最高。本研究为进一步揭示GbVIN1基因可能对棉纤维伸长具有重要作用提供了一定的理论依据。

According to the preliminary cotton fiber development transcriptional data and spectral analyses, we cloned a homologous gene from cotton （Gossypium barbadense L.） cultivar ‘Xinhai21＇ using reverse transcription PCR method, which was named as Gb VINI. The gene had an open reading frame of 1 938 bp, encoding 645 amino acids. The multiple sequence alignment analysis showed that the protein was highly conserved, which had the -NDPNG- and -WECVD- conservative sequence motif of GH32 family. The phylogenetic tree analysis indicated that the GbVIN1 gene and the GhVIN1 gene were in the same branch of the evolutionary tree. Real time fluorescence quantitative PCR analysis demonstrated that Gb VIN1 gene expressed in different periods of cotton fiber development, and the highest expression was found in 5 DPA and 10 DPA fibers. The study could provide a theoretical basis for further revealing that Gb VINI gene might play an important role in cotton fiber elongation.