高压氧对缺氧缺血性脑损伤新生大鼠JAK/STAT3信号通路的影响

Effects of hyperbaric oxygen on JAK/STAT3 signaling pathway in neonatal rats with hypoxic-ischemic brain damage

目的探讨高压氧对缺氧缺血性脑损伤新生大鼠JAK/STAT3信号通路的影响。方法选取36只7日龄SD新生大鼠,随机分为3组:假手术组(n=12),缺氧缺血性脑损伤组(n=12),高压氧治疗组(n=12)。采用Rice法制作新生大鼠缺氧缺血性脑损伤模型,假手术组大鼠分离左颈总动脉后不结扎直接缝合皮肤,高压氧治疗组大鼠在模型制作成功后,给予高压氧治疗,1h/d,连续7d。测定各组大鼠体质量增长率与左/右脑重量比值;应用TUNEL法检测脑组织神经细胞凋亡情况,Western blot方法与real time PCR方法分别检测各组新生大鼠脑组织JAK与STAT3蛋白与mRNA的表达。结果高压氧治疗可使缺氧缺血性脑损伤新生大鼠的体质量增长率和左/右脑重量比值增加(P<0.05)。与假手术组相比,缺氧缺血性脑损伤组新生大鼠脑组织的神经细胞凋亡数目、JAK与STAT3蛋白与mRNA的表达均显著升高(P<0.01);与缺氧缺血性脑损伤组相比,高压氧治疗组新生大鼠脑组织的神经细胞凋亡数目、JAK与STAT3蛋白与mRNA的表达均显著降低(P<0.01)。结论高压氧减轻缺氧缺血性脑损伤新生大鼠的脑组织损伤可能与抑制JAK/STAT3信号通路相关。

Objective To investigate the effect of hyperbaric oxygen on JAK/STAT3 signaling pathway in neonatal rats with hypoxic-ischemic brain damage. Methods A total of 36 neonatal SD rats aged 7 days were randomly divided into 3 groups： sham group（n=12）, hypoxic ischemic brain damage group（HIBDgroup, n=12）, hyperbaric oxygen treatment group（HBOgroup, n=12）. The hypoxic ischemic brain damage model of neonatal rats was made by Rice method. The rats in the sham operation group were separated from the left common carotid artery without ligating and sutured the skin directly. The rats in the hyperbaric oxygen therapy group were treated with hyperbaric oxygen, 1 h/d, and 7 d after the model was made. The rate of body weight and the ratio of left/right brain was measured in each group. Apoptosis of brain tissue was detected by TUNEL method. The expression of JAK/STAT3 protein and mRNA were detected by Western Blot method and real time PCR method respectively. Results Hyperbaric oxygen therapy could increase the body weight and left/right brain weight of neonatal rats with hypoxic-ischemic brain damage（P〈0.05）. Compared with the sham group, the number of neuron apoptosis, the expression of JAK and STAT3 protein and mRNA were significantly increased in the neonatal rats of hypoxic ischemic brain damage group（P〈0.01）. Compared with the hypoxic ischemic brain damage group, the number of neuron apoptosis, the expression of JAK and STAT3 protein and mRNA were significantly decreased in the neonatal rats of hyperbaric oxygen therapy group（P〈0.01）. Conclusion HBO attenuated brain injury in neonatal rats with hypoxicischemic brain damage, which might be related to inhibiting JAK/STAT3 signaling pathway.