ICOS信号与日本血吸虫感染小鼠Th9细胞极化的关系

Relation between ICOS signaling and Th9 cell polarization in mice infected with Schistosoma japonicum

目的检测可诱导共刺激分子(ICOS)在日本血吸虫感染小鼠脾脏Th9细胞中的表达水平,探讨ICOS信号与Th9细胞极化之间的关系。方法以日本血吸虫尾蚴感染小鼠,分别在感染后0、4、7、9周和12周,应用流式细胞术检测IL-9+细胞在CD4+T细胞中的比例,以及ICOS+细胞在Th9细胞中的比例。结果与0周比较,感染后4、7、9、12周,小鼠CD4+T细胞中Th9细胞的比例显著增高(P <0.05),并且Th9细胞ICOS的表达也明显上调(P <0.05)。结论在日本血吸虫感染中,ICOS信号对Th9细胞的极化可能具有调控作用。

Objective To detect the expression level of ICOS on Th9 cells in mice infected with Schistosoma japonicum,and investigate the relation between ICOS signaling and Th9 cell polarization. Methods Twenty.five mice with S. japonicum infec.tion were used as models. IL.9＋cells in CD4＋ T cells and ICOS＋ cells in Th9 cells of the mice were detected by flow cytometry 0,4,7,9 weeks and 12 weeks after the infection. Results Compared with that 0 week after the infection,the proportion of Th9 cells in CD4＋ T cells of the mice significantly increased 4,7,9,12 weeks after the infection（all P〈0.05）,and the proportion of ICOS＋ cells in Th9 cells also markedly improved（P〈0.05）. Conclusion In S. japonicum infection,the ICOS signaling may have a regulatory effect on Th9 cell polarization.