葡萄白粉病潜在生防菌的筛选

Screening of Potential Biocontrol Agents for Grape Powdery Mildew Caused by Uncinula necator

[目的]筛选葡萄白粉病潜在生防菌。[方法]分别对4种链霉菌、WCS417r及转phl基因菌株5株和WCS358r及其转基因菌株5株分别进行离体叶片试验。[结果]从4株链霉菌中,筛选出2株有较好防治效果的菌株,分别比对照降低了53.3和60.0百分点;从WCS417r及转phl基因菌株共5株菌株中筛选出3株菌株,从WCS358r及转基因菌株共5株菌株中筛选出2株菌株,分别比对照降低了65.0、70.0、80.0、40.0和40.0百分点,明显优于对照。[结论]WCS358r和WCS417r的转基因菌株具有增强防病的效果。

[Objective] To screen potential biocontrol agents for grape powdery mildew caused by Uncinula necator. [Method] Four strains of Streptomyces, 5 strains of WCS417r and transphl and 5 strains of WCS358r and thir transgenic strains were tested separately in vitro. [Result] From 4 strains of Streptomyces, 2 strains with good control effect were selected,which decreased by 53.3 and 60.0 percentage points respectively compared with the control. 3 strains were selected from 5 strains of WCS417r and phl transgenic strain,and 2 strains were selected from 5 strains of WCS358r and transgenic strain,which were decreased by 65.0,70.0,80.0,40.0 and 40.0 percentage points,respectively,compared with the control. [Conclusion]Transgenic strains of WCS358r and WCS417r have enhanced disease prevention effect.