青花菜FLC1基因多态性分析与克隆

Cloning and Sequence Analysis of FLCI in Broccoli

采用11对与十字花科春化和开花相关的分子标记，对青花菜不同熟性品系基因组DNA进行PCR扩增分析发现，FLC1基因存在多态性。进一步的分析表明该多态性与品系间的熟性性状相关性不大。通过RACE技术克隆两个熟性差异较大的青花菜FLC1基因，并进行了序列对比表明，两个青花菜FLC1基因之间存在单个基因的差异，但根据此设计的引物未能发现其与熟性性状之间的相关性。

Amplications of polymerase chain reactio （PCR） with 11 markers about vernalization and flowering came from cruciferae were conducted in broccoli lines, which has different date of maturity. Results showed that the FLC1 gene is polymorphic. Further analysis indicated that the polymorphism was not related to the maturity strains. Clone of the FLC1 gene of two broccoli lines through RACE method was also carded out, and sequence alignment suggested the differences of individual bases between two lines existed, but they had no relationship with maturity traits by PCR.