不同运载体系对虾青素酯生物利用度的影响研究

Study on the effect of different delivery systems on the bioavailability of esterified astaxanthins

目的:研究不同运载体系对虾青素酯在模型小鼠体内消化吸收的影响情况。方法:选用Balb/c小鼠作为模型动物,通过给予模型小鼠灌胃不同运载体虾青素酯,研究虾青素酯在小鼠体内的消化、排泄情况;并通过测定单次灌胃不同运载体系虾青素酯后小鼠血液中的虾青素浓度-时间曲线,考察其在模型动物体内的代谢动力学和生物利用率。结果:不同运载体系中虾青素酯生物可接受率和AUC0-72值由高到低依次为:虾青素酯脂质体、微乳液、微胶囊和油剂;虾青素酯脂质体和微乳液在小鼠血清中达最大吸收峰值均在7h,而微胶囊在9h,油剂在12h,其虾青素的最大吸收浓度依次分别为0.72μg/mL、0.55μg/mL、0.36μg/mL和0.21μg/m L;上述结果表明,通过设计运载体系可以有效的提高虾青素酯的消化吸收效率和生物利用度。结论:为虾青素酯作为功能因子在食品中的高效应用提供了科学依据。

In this paper, digestibility and absorbability of astaxanthin esters in different delivery systems was studied using Balb/c mice as model animals. The digestion and excretion of astaxanthin esters in mice were evaluated by single-dose oral gavage of esterified astaxanthins under different delivery systems. Then the astaxanthin concentration-time curve was determined, and pharmacokinetics and bioavailability of astaxanthin in blood of mice were investigated. The results showed that the ascending order of absorption capacities of astaxanthin esters in different delivery systems was ： liposome 〉 microemulsion 〉 microcapsule 〉 oleoresin. The concentration of astaxanthin in plasma reached a maximum （Cmax） at 7 h after feeding esterified astaxanthins liposome and esterified astaxanthins microemulsion, reached a maximum at 9 h after feeding esterified astaxanthins microcapsule and reached a maximum at 12h after feeding esterified astaxanthins oleoresin. The Cmax in plasma was 0.721μg/mL, 0.55μg/mL, 0.361μg/mL and 0.211μg/mL, respectively. These results could provide an important evidence for effectively improving bioavailability of astaxanthin esters by designing delivery system. Furthermore, this paper can supply a scientific basis for using esterified astaxanthins as bioactive components in food products.