分光光度法测定黑米花青素含量的不确定度评定

Uncertainty evaluation for determining concentration of anthocyanins in black rice by spectrophotometry

评定分光光度法测定黑米花青素含量的不确定度。建立相应的数学模型,并对影响模型中各因素的不确定度来源进行分析、计算和评定。结果表明样品测定重复性带来的不确定度和样品液中花青素浓度的不确定度是合成不确定度的主要来源;按照JJF1059-2012《测量不确定度评定与表示》对各不确定度分量合成和扩展,当取置信概率95%,最终得到测量的扩展不确定度为0.0972g/100g(k=2)。本方法对分光光度法测定黑米中花青素含量结果的不确定度评定具有参考作用。

In order to evaluate the uncertainty of anthocyanin＇ s content in black rice detected by spectrophotometry, the mathematical model was established. The uncertainties of factors in mathematical model were analyzed, calculated and evaluated. The results showed that the uncertainties due to the repeatability of sample determination, and uncertainty of anthocyanin＇s concentration in the sample solution were the main sources of U （X） . According to JJF1059-2012, the component uncertainties were synthesized and extended. An expanded uncertainty 0.0972g/100g was obtained at confidence probability 95% and coverage factor 2. This method provides reference for the uncertainty evaluation of anthocyanin＇ s content in black rice by spectrophotometry.