隔药饼灸对高脂血症合并动脉粥样硬化兔血清TNF-α、INF-γ含量的影响

Effect of Herbal Cake-Separated Moxibustion on the Expression of Serum TNF-α and INF-γ in Rabbits with Hyperlipidemia and Atherosclerosis

目的观察隔药饼灸对高脂血症合并动脉粥样硬化兔血清中肿瘤坏死因子α(TNF-α)、干扰素γ(INF-γ)含量的影响,探讨其抗动脉粥样硬化作用的机制。方法 40只新西兰大耳白兔随机分为5组,每组8只,即正常组、模型组、隔药饼灸组、直接灸组、辛伐他汀组。通过高脂饲料喂养建立高脂血症合并动脉粥样硬化兔模型,待造模成功后,除正常组外,其余四组均予以相应干预,每天1次,连续干预4周后,采用酶联免疫吸附法(ELISA)测定兔血清中TNF-a、IFN-γ。结果与正常组比较,模型组、直接灸组、辛伐他汀组血清TNF-α、IFNγ的含量升高,且差异具有统计学意义(P<0.01或P<0.05);与模型组相比,直接灸组、辛伐他汀组、隔药饼灸组中血清TNF-α、IFNγ的含量均明显下降,差异具有统计学意义(P<0.01或P<0.05);隔药饼灸组与正常组相比较,隔药饼灸组的血清IFNγ含量有升高趋势,但差异无统计学意义(P>0.05),TNF-α与正常组相比含量增多,差异具有统计学意义(P<0.05)。结论隔药饼灸对动脉粥样硬化兔血清相关炎性因子TNF-α、IFN-γ的影响是隔药饼灸抗动脉粥样硬化作用机制之一。

Objective To observe the efleet of herbal cake-separated moxibustion on the expression of tumor necrosis factor alpha （TNF-α and interferon ganmm （INF-γ） in the sermn of rabbits with hyperlipidemia and atheroselerosis, and to investigate the meehanisnl of its anti-atheroselerotie effeet, Methods A total of 40 New Zealand white rabbits were randomly divided into five groups, namely blank control group （normal group）, model group, herbal cake - separated moxibustion group, direct moxibustion group, and simvastatin group, with eight rabbits in each group. A hyperlipidemia and atheroselerosis rabbit model was established by feeding high-fat diet to the animals. After successful modeling, all the five groups, except the blank control group, received corresponding treatment once a clay for four successive weeks; then enzyme-linked immunosorbent assay was used to determine the levels of TNF-α and IFN-γ in the serum of the rabbits. Results The expression levels of TNF-α and IFN-γ in the sermn increased significantly in the model group, the direct moxibustion group, and the simvastatin group compared with those in the nomlal group （P〈0.01 or P〈0.05）; the sermn levels of TNF-α and IFN-γ decreased significantly in the direct moxibustion group, the simvastatin group, and the herbal cake - separated moxibustion group eompared with that in the model group （P〈0.01 or P〈0.05）; eompared with the normal group, the herbal eake-separated moxibustion group had a signifieantly inereased expression level of serum TNF-α （P〈0.05）, but there was no signifieant differenee between the two groups in the serum level of IFN-y despite an inereasing trend in the herbal eake- separated moxibustion group （P〉O.05）. Conclusion The efIeet on relevant inflammatotT faetors （TNF-α and IFN-γ） in the serum of atheroselerotie rabbits is one of the anti-atheroselerotie meehanisms of herbal eake-separated moxibustion.