摘要
The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued ef forts to improve the Faecal Contamination Source Tracking(FCST) techniques such as Microbial Source Tracking(MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' eff ects in tracking the major sources of faecal contamination in a freshwater system(Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.
The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued ef forts to improve the Faecal Contamination Source Tracking(FCST) techniques such as Microbial Source Tracking(MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' eff ects in tracking the major sources of faecal contamination in a freshwater system(Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.
作者
Oscar Omondi DONDE
田翠翠
肖邦定
Oscar Omondi DONDE;TIAN Cuicui;XIAO Bangding(Key Laboratory of Algal Biology of Chinese Academy of Sciences-Lake Restoration Research Group,Institute of Hydrobiology Chinese Academy of Sciences,Wuhan 430072,China;International College,University of Chinese Academy of Sciences,Beijing 100049,China;Egerton University,Department of Environmental Science,P.O.Box 536-20115,Egerton-Kenya)
基金
Supported by the National Natural Science Foundation of China(Nos.31670465,31370504)
关键词
粪便污染源
海洋学
发展现状
微生物源
assay
contamination
faecal bacteria indicator
source tracking
water quality