基于网络药理学方法研究补肾活血方治疗肾纤维化药效物质基础及分子作用机制

Pharmacodynamic material basis and molecular mechanism of Bushen Huoxue prescription in treatment of renal fibrosis using network pharmacology

目的:探讨补肾活血方治疗肾纤维化的药效物质基础及分子作用机制。方法:基于网络药理学理论框架,结合ADME性质筛选、靶点预测、网络构建及KEGG通路富集分析等方法进行研究。结果:补肾活血方中5味药材共包含491个化合物,其中具有良好的ADME性质的188个潜在活性成分中,共有136个活性成分及其对应的59个靶标与补肾活血方治疗肾纤维化密切相关。通过构建"药材-活性成分-靶标"网络及对网络进行分析,共获得19个关键化合物和5个关键靶标。此外,在对靶标进行KEGG通路分析时,共获得19条与补肾活血方治疗肾纤维化相关的通路,其中,PI3K-Akt信号通路、MAPK信号通路、HIF-1信号通路为主要通路。结论:补肾活血方可通过多成分、多靶标及多通路的作用模式治疗肾纤维化。本研究为补肾活血方治疗肾纤维化的药效物质及潜在作用机制研究提供了一种新的研究思路,为该方剂的进一步升级改造及临床研究提供了必要的理论依据。

Objective： Investigate the pharmacodynamic material basis and molecular mechanism of Bushen Huoxue prescription in treatment of renal fibrosis. Methods： Based on the theoretical framework of network pharmacology, ADME screening, target prediction, network construction and KEGG pathway enrichment analysis were applied for this work. Results： There were 491 compounds in the 5 medicinal herbs of Bushen Huoxue prescription. In these compounds, 188 compounds were as potential active components with good ADME properties, and 136 active compounds and corresponding 59 targets were closely related to Bushen Huoxue prescription in the treatment of renal fibrosis. By constructing and analyzing the active ingredients-targets network, 19 key compounds and 5 key targets were obtained. In addition, the KEGG pathway analysis showed that 19 pathways related to the Bushen Huoxue prescription for kidney fibrosis, and PI3K-Akt pathway, MAPK signaling pathway and HIF-1signaling pathway were the major pathways. Conclusion： Bushen Huoxue prescription can treat renal fibrosis through multi-component, multi-target and multi-pathway. This study provides a new idea for the study of the pharmacodynamic substances and potential mechanism of Bushen Huoxue prescription in the treatment of renal fibrosis, and provides the necessary theoretical basis for the further upgrading and clinical research of the prescription.