摘要
2016年从北京市10个区采集317份玉米瘤黑粉病样品,选择核糖体RNA内转录间隔区的ITS rDNA和28S rDNA基因作为持家基因,通过单核苷酸多态性技术(Single Nucleotide Polymorphism,SNP)分析北京市10个区玉蜀黍黑粉菌的遗传多样性。ITS rDNA基因分析表明,有3个菌株的序列存在1个颠换突变位点,产生两种单倍型,采用邻接法构建的系统发育树将317个菌株分为2个群体; 28S rDNA基因分析表明,有54个菌株的序列存在1个颠换突变位点,产生两种单倍型,采用邻接法构建的系统发育树将317个菌株分为2个群体。中性检测表明北京市10个区的玉蜀黍黑粉菌符合中性进化模型。试验结果表明北京市10个区的玉蜀黍黑粉菌遗传多样性不高,遗传分化不明显。
We collected 317 Ustilago maydis samples from 10 suburban districts in Beijing, and used ITS rDNA and 28S rDNA gene of internal transcribed spacer in ribosome RNA as housekeeping gene to analyze their genetic diversity through SNP. The analysis of ITS rDNA indicated that 1 transversion locus was found in 3 strains and detected 2 haplotypes, Neighbor joining（NJ） tree divided the 317 strains into 2 clusters; The analysis of 28S rDNA indicated that 1 transversion locus was found in 54 strains and detected 2 haplotypes, NJ tree divided the 317 strains into 2 clusters. Neutrality test showed that the evolution of U. maydis was according with neutral theory. The genetic diversity of U. maydis from suburban districts in Beijing was not high, genetic difference was insignificant.
作者
李超
金海伦
高利
刘太国
刘博
陈万权
LI Chao1,2, JIN Hai-lun1,2 , GAO Li1 , LIU Tai-guo1 , LIU Bo1, CHEN Wan-quan1(1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2College of Life Science, Yangtze University, Jingzhou 434025, China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2018年第5期632-639,共8页
Acta Phytopathologica Sinica
基金
国家自然科学基金委国际(地区)合作与交流项目(31761143011)
国家自然科学基金面上项目(31571965)
北京市自然基金面上项目(6162022)
中国农科院优青引导项目(Y2017JC09)
国家重点研发计划(2016YFC1201200)
