苹果褪绿叶斑病毒辽宁分离物生物学和基因组研究

Biology and complete genome of Apple chlorotic leaf spot virus Liaoning isolate

苹果褪绿叶斑病毒（Apple chlorotic leaf spotvirus，ACLSV）属于乙型线形病毒科（Betaflexiviridae）纤毛病毒属（Trichovirus），基因组为正义单链RNA。含有约7500个核苷酸。编码3个部分重叠的开放阅读框。目前从GenBank数据库可以检索到19个ACLSV分离物的基因组序列。

In the present work, we studied the genomic and biological properties of Apple chlorotic leaf spot virus （ACLSV） isolate XC-HF. The leaves of Malus domestica cv. Hanfu collected from Xingcheng, Lianoning province were used as plant material. The samples were subjected to RT-PCR and RACE techniques to amplify the complete genome of ACLSV XC-HF, followed by sequencing. The genome of ACLSV XC-HF consists of 7 557 nucleotides, encoding three overlapping open reading frames （ORFs） and shared nucleotide identities of 68.9%-84.4% with the other 19 ACLSV isolates published in GenBank. Phylogenetic analysis based on the complete genorne sequences indicated that ACLSV XC-HF was located in the group JB. Recombination analysis suggested a recombinant event at 1-338 nts of ACLSV XC-HF. The ACLSV XC-HF was inoculated to Chenopodium amaranticolor by sap inoculation and to Malus sylvestris cv. R12740-7A by double budding, respectively, and induced symptoms of chlorosis and slight leaf roll on infected plants.