摘要
肿瘤是一类与细胞周期密切相关的疾病。流式细胞术对肿瘤细胞周期的分析可以观察到肿瘤细胞倍体的变化,有助于肿瘤的早期发现和治疗。区分肿瘤细胞所处于的细胞周期,明确地了解每个时相所占细胞周期的比例,对于肿瘤药物的研发具有重要意义。该文着重对G0期和G1期,G2期和M期,以及S期细胞的测定方法进行归纳、总结,并对这些方法的优缺点及适用条件进行阐述和总结,为肿瘤药物的研发及基础研究等方面的科研工作提供参考。
Tumor is a kind of disease that is closely related to cell cycle. The analysis of tumor cell cycle by flow cytometry can observe the change of tumor cell ploidy, which is helpful for early detection and treatment of tumor. It is of great significance to distinguish the cell cycle of tumor cells and to understand the proportion of cell cycle in each phase. In this paper, the determination methods on the G0 and G1 phase, the G2 and M, and S phase are summarized, and at the same time we expounds the advantages and disadvantages of these methods and the suitable conditions., which provide an reference for cancer drug research and pharmacological research.
作者
高世勇
谢明宏
谭慧心
李丹
李琳
吴爽
安娜
GAO Shiyong1, 3, XIE Minghong1, 3, TAN Huixin2, LI Dan1, 3, LI Lin1,3, WU Shuang1, 3, AN Na1, 3(1. Institute of Materia Medica of Harbin Commerce University, Harbin 150076, China 2. Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China ;3. Key Laboratory for Cancer Prevention and Anticancer Drug Research of Heilongjiang, Harbin 150076, China)
出处
《药物评价研究》
CAS
2018年第8期1374-1379,共6页
Drug Evaluation Research
基金
哈尔滨商业大学研究生创新科研资金项目(YJSCX2017-456HSD)
哈尔滨商业大学科研团队计划项目(2016TD002)
黑龙江省卫生计生委科研课题(2017-132)
哈尔滨市科技创新人才研究专项资金项目(2017RAXXJ085)
黑龙江省教育厅科学技术研究项目(12541571)
黑龙江省高等学校创新团队建设计划项目(2014TD009)
黑龙江省级领军人才梯队后备带头人资助项目
关键词
肿瘤
细胞周期
测定方法
tumor
cell cycle
determination method
