白头翁汤对链脲佐菌素诱导的糖尿病肝损伤的保护作用研究

Protective effect of Pulsatillae Decoction on STZ-induced diabetic hepatic injury

目的观察白头翁汤对链脲佐菌素(STZ)诱导的I型糖尿病小鼠肝损伤的治疗效果。方法单次ip STZ(180 mg/kg)建立1型糖尿病小鼠模型,对照组给予等量的柠檬酸缓冲液。挑选血糖值≥15 mmol/L的小鼠随机分为模型组、二甲双胍组及白头翁汤低、中、高剂量(生药2.5、5.0、10.0 g/kg)组,对照和模型组给予等体积生理盐水,连续ig给药4周。末次给药24 h后,称各组小鼠体质量,测空腹血糖;试剂盒法检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平,检测血清及肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平,HE染色观察肝组织病理组织学损伤。结果给药4周后,与模型组比较,白头翁汤中、高剂量组动物体质量显著升高、空腹血糖值显著降低(P<0.05、0.01);中、高剂量组血清ALT、AST、ALP、TNF-α、IL-6水平显著下降(P<0.05、0.01、0.001);中、高剂量显著降低血清及肝组织中MDA含量,高剂量显著提升血清及肝组织中SOD活力,中、高剂量显著增加血清GSH-Px水平,高剂量显著增加肝组织GSH-Px水平(P<0.05、0.01)。HE染色显示白头翁汤可以改善肝脏病理组织学损伤。结论白头翁汤可以有效改善STZ诱导的糖尿病肝损伤,机制与减轻脂质过氧化、增强机体清除自由基能力以及抗炎作用有关。

Objective To investigat the protective effect of Pulsatillae Decoction on streptozocin（STZ） induced Type I diabetes. Methods The diabetic model was induced with a single dose of 180 mg/kg STZ. The fasting blood glucose over 15 mmol/L was considered as diabetes. The diabetic mice were randomly divided into five groups： model group, metformin group, Pulsatillae Decoction low, medium, and high dose groups. The mice were ig administrated with the drugs for continuous four weeks, then the body weight, fast blood glucose, the levels of ALT, AST, ALP, TNF-α, and IL-6 in serum were detected. The levels of MDA, SOD, GSH-Px in serum and hepatic tissues were also examined. The histopathological changes were observed by HE staining. Results After a four week treatment, Pulsatillae Decoction medium and high dose groups significantly increased the body weight, decreased the fast blood glucose（P〈0.05 and 0.01）, reduced the levels of ALT, AST, ALP, TNF-α, and IL-6（P〈0.05, 0.01, and 0.001）, attenuated the levels of MDA, SOD, and GSH-Px in serum and hepatic tissues（P〈0.05 and 0.01）. The results of HE staining also showed that Pulsatillae Decoction was able to relive the histopathological alteration. Conclusion Pulsatillae Decoction could ameliorate the STZ-induced diabetic hepatic injury, possibly via the scavenging Oxygen free radical, anti-oxidative, and anti-inflammatory activities.