参芪益智颗粒对APP/PS1转基因小鼠血清MMP2和VEGF含量的影响

Effect of Shenqi Yizhi Granule on Serum MMP2 and VEGF Levels in APP/PS1 Transgenic Mice

目的:探究参芪益智颗粒对APP/PS1转基因小鼠学习认知功能的影响及血清中MMP2和VEGF含量的影响。方法:以APP/PS1双转基因小鼠为研究载体,参芪益智颗粒干预180天。采用Morris水迷宫、避暗和开场试验评价小鼠行为学变化,HE染色观察小鼠海马CA1区病理形态学变化,ELISA法检测血清中MMP2和VEGF的含量。结果:与空白组比较,模型组小鼠隐藏平台实验逃避潜伏期显著延长(P <0.05),空间探索实验进入平台象限次数和平台象限所占时间百分比明显减少(P <0.05);海马CA1区神经细胞数量减少,排列紊乱,核固缩深染明显;血清中MMP2和VEGF的含量明显增高(P <0.05)。与模型组比较,参芪益智颗粒可缩短该模型小鼠的逃避潜伏期(P <0.05),增加进入平台次数和进入平台象限百分比(P <0.05),降低血清中MMP2和VEGF的含量(P <0.05)。结论参芪益智颗粒能提高APP/PS1双转基因小鼠学习记忆能力,降低血清中MMP2和VEGF的含量。

Objective： To explore the effects of Shenqi Yizhi Granule on the cognitive function and the expression of MMP-2 and VEGF in serum of APP/PS1 transgenic mice. Methods： Taking APP/PS1 double transgenic mice as the research carrier, Shenqi Yizi granule was used to interven for 180 days. The learning and remembering ability was tested by Morris water maze, passive avoidance test, and open field. The pathomorphism of the hippocampus CA 1 region of mice was tested by HE staining and electron microscopy. The concentration of MMP-2 and VEGF in serum was tested by ELISA. Results： Compared with the blank group, the escape latency of the model group was significantly increased （P 〈 0.05）, and the frequency of the entering medium ring was obviously decreased （P 〈 0.05）. The number of nerve cells in the hippocampal CAI area was reduced, and the arrangement was disordered. The nuclear pyknosis was deeply stained, and the ultrastructure of nerve cells was abnormal. The concentration of MMP-2 and VEGF in serum was significantly increased （P 〈 0.05）. Compared with the model group, the escape latency of treatment group was decreased （P 〈 0.05） and the frequency of the entering middle ring was increased （P 〈 0.05）. The concentration of MMP-2 and VEGF in serum was decreased （P 〈 0.05）. Conclusion： Shenqi Yizhi Granule could improve the ability of learning and memory of APP/ PS1 double transgenic mice, and decrease the concentration of MMP-2 and VEGF in serum.