苦艾提取物对树突状细胞成熟及其功能的影响

Effects of Artemisia absinthium L. extracts on the maturation and function of dendritic cells

目的 检测苦艾（Artemisia absinthium L.）提取物对树突状细胞（dendritic cell,DC）成熟和功能的影响.方法 制备苦艾水提物（Artemisia absinthium water extract,AAW）和醇提物（Artemis-ia absinthium ethanol extract,AAE）,用含不同多糖浓度AAW（5μg/ml、50μg/ml、150μg/ml）和含不同黄酮浓度AAE（0.6μg/ml、3μg/ml、6μg/ml）处理DCs,通过流式细胞术检测AAW及AAE对DC活性、抗原吞噬能力和DC成熟的影响.酶联免疫吸附法检测DC分泌的细胞因子水平,Western blot检测核因子κB（nuclear factor kappa B,NF-κB）、丝裂原活化蛋白激酶（mitogen-activated protein kinase,MAPK）和酪氨酸激酶2/信号转导子和转录激活子3（janus kinase/signal transducer and activator of transcription,JAK2/STAT3）信号通路关键分子的活化水平.结果 AAW促进DC的成熟,显著降低DC的抗原吞噬能力,提高白细胞介素-12p40（interleukin-12p40,IL-12p40）和肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）的分泌水平.AAE显著增强DC表面共刺激分子的表达,降低DC的抗原吞噬能力,对DC的细胞因子水平没有影响,但是显著降低了细菌脂多糖（lipopolysaccharide,LPS）诱导的TNF-α、IL-12p40和IL-6水平.进一步研究表明,AAW和AAE能激活p38、细胞外信号调节蛋白激酶（extra-cellular signal regulated kinase,ERK）、IKKα/β、NF-κBp65及JAK2的磷酸化水平,AAE还能激活c-Jun氨基末端激酶（c-Jun N-terminal kinase,JNK）的磷酸化水平,但是AAE与LPS联用抑制了LPS激活的NF-κB抑制蛋白（inhibitor of NF-κB,IκB-α）、IKKα/β、NF-κBp65、p38、ERK及JAK2的磷酸化.结论 AAW具有免疫增强作用,AAE具有抗炎作用.

Objective To investigate the effects of Artemisia absinthium L. （ A. absinthium L. ） extracts on the maturation and function of dendritic cells （DCs）. Methods A. absinthium water （AAW） and ethanol （ AAE） extracts were prepared. DCs were separated into several groups and treated with differ-ent concentrations of AAW （containing 5, 50 and 150μg/ml of polysaccharide） and AAE （containing 0. 6, 3 and 6 μg/ml of flavonoid） , respectively. Cell viability, antigen phagocytosis and maturation of DCs were detected by flow cytometry. Levels of cytokines were analyzed by ELISA. Western blot assay was performed to analyze the activation of key molecules in NF-κB, mitogen-activated protein kinase （ MAPK） and janus kinase/signal transducer and activator of transcription （ JAK/STAT） signaling pathways. Results AAW promoted the maturation of DCs, significantly decreased antigen phagocytosis and increased cytokine produc-tion （IL-12p40 and TNF-α）. AAE significantly enhanced the expression of co-stimulatory molecules on the surface of DCs and decreased antigen phagocytosis, but had no significant effect on cytokine production. Mo-reover, AAE significantly inhibited the LPS-induced expression of TNF-α, IL-12p40 and IL-6. Further anal-ysis revealed that AAW and AAE could activate the phosphorylation of p38, extra-cellular signal regulated kinase （ERK）, IKKα/β, NF-κBp65 and JAK2. Besides, AAE could activate the phosphorylation of c-Jun N-terminal kinase （ JNK ） and inhibit the LPS-induced phosphorylation of inhibitor of NF-κB （ IκB-α） , IKKα/β, NF-κBp65, p38, ERK and JAK2. Conclusion AAW could enhance immunity and AAE could inhibit inflammation.