抑郁症患者肠道菌群研究

Intestinal microbial community in patients with depression

目的研究抑郁症患者肠道微生物群落分布情况。方法选择2017年1月-2018年1月在本院临床心理科就诊的确诊为抑郁症的患者79例,以及同时期在本院体检的健康者80例。收集患者新鲜粪便,利用16SrRNA基因测序技术分析患者肠道菌群。结果对159例粪便标本进行16SrRNA基因测序,共获得1 276 841条有效16SrRNA基因序列,抑郁组Shannon指数明显高于对照组。在门水平,共发现20个细菌门,抑郁患者肠道菌群丰度前3位的分别是厚壁菌门、拟杆菌门、梭杆菌门;在科水平,前3位主要为拟杆菌科、普雷沃氏菌科和瘤胃菌科;在属水平,前3位分别是多形杆菌属、普氏菌属、另枝菌属。对照组肠道菌群丰度前3位的分别是厚壁菌门、拟杆菌门、梭杆菌门;在科水平,前3位主要是拟杆菌科,普雷沃氏菌科和瘤胃菌科;在属水平,前3位分别是多形拟杆菌属、普氏菌属、另枝菌属。抑郁组多形杆菌属、普氏菌属、另枝菌属、栖粪杆菌属、考拉杆菌属丰度明显低于对照组,抑郁组毛螺菌属、副杆菌属、布劳特氏菌属、巨单胞菌属丰度明显高于对照组。抑郁组拟杆菌属和栖粪杆菌属丰度与SDS评分成负相关,毛螺菌属丰度与SDS评分成正相关。结论抑郁症患者病情严重程度与抗炎性细菌拟杆菌属和栖粪杆菌属丰度成反比,与毛螺菌属丰度成正比。

Objective To explore the distribution of intestinal microflora in patients with depression.Methods A total of 79 patients with depression diagnosed in the clinical psychology department of our hospital from January 2017 to February 2018,and 80 healthy individuals examined in our hospital at the same time were selected.The samples of fresh feces were collected for analysis of intestinal flora using 16 SrRNA gene sequencing technology.Results The 16 SrRNA gene sequencing resulted in 1 276 841 effective16 SrRNA gene sequences from the 159 stool samples.The Shannon index of the depression group was significantly higher than that of the control group.A total of 20 bacterial phyla were found,with the top three abundant phyla being Firmicutes,Bacteroides and Fusobacteria.At the level of family,the first three were Bacteroides,Prevoaceae and Rumen bacteria.At the genus level,the first three were Polymorpha,Prevotellaand Alistipes.The same results were seen in the control group,but the abundances of Polymorpha,Prevotella,Alistipes,Faecalibacteriaand Phascolarctobacteria were lower while those of Lachnospiraceae,Parabacteroides,Blautiaand Megamonas were higher in the depressin group than in the control group,respectively.The abundances of Bacteroides and Faecalibacteriain the depression group were negatively correlated with the SDS score,while that of Lachnospiraceae was positively correlated with the SDS score.Conclusion The severity of depression is inversely proportional to the abundances of the anti-inflammatory bacteria Bacteroides and Faecalibacteria,while proportional to the abundance of Lachnospiraceae.