黔产石韦排石作用活性部位的研究

Lithagogue effects of Pyrrosia lingua from Guizhou province on experimental renal calculus in rats

筛选黔产石韦药材排石作用的活性部位并对活性部位进行研究探讨药效化合物。采用1％乙二醇和2％氯化铵喂饲法诱导的大鼠肾结石模型，观察黔产石韦药材不同极性部位排石的作用，检测各组大鼠尿液中尿钙、尿草酸的含量，肾组织中肾钙、肾草酸和超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）、肾组织中结晶沉积或结石形成的情况及血清中的肌酐（Cr）、尿素氮（BUN）含量；基于LC—MS技术对活性部位的提取物进行分析推断药效化合物。黔产石韦药材石油醚部位，二氯甲烷部位组均能降低。肾草酸和肾钙离子浓度，升高尿草酸和尿钙量，对大鼠肾结石的形成具有显著的抑制作用，并均能显著升高肾组织SOD，CAT活性，且还能明显减少MDA；基于LC-MS分析得出石油醚，二氯甲烷部位的化合物中咖啡因、柠檬酸和酒石酸具有排石作用。黔产石韦的石油醚部位和二氯甲烷部位均为中剂量组具有较好的防治结石作用，其机制与抗氧化、减少草酸钙结晶沉积、促进草酸钙结晶排出有关，且咖啡因、酒石酸和柠檬酸具有排石作用。

To screen the active fractions with lithagogue effects of Pyrrosia lingua from Guizhou province and preliminarily investigate its mechanism. The rats were fed with 1% ethylene glycol and 2% ammonium chloride to establish the uephrolithiasis models, which were used to evaluate thelithagogue effects of different polar fractions of P. lingua from Guizhou province. The level of urinary calcium and oxalic acid in urine, renal calcium, oxalic acid, superoxide dismutase （SOD）, catalase （CAT） and malondialdehyde （MDA） in renal tissues, as well as crystalline deposit and lithogenesis in renal tissues and the levels of creatinine（Cr） and blood urea nitrogen （BUN） in the serum were detected. The effective compounds were inferred from the analysis of active fractions extract based on LC-MS technology. Petroleum ether fraction and dichloromethane fraction of P. lingua from Guizhou province can reduce renal oxalic acid and renal calcium concentration, increase urinary oxalic acid and urine calcium, with significant inhibitory effect on the formation of renal calculus in rats, significantly increase SOD and CAT activities in renal tissues, and significantly reduce MDA levels. LC-MS analysis showed that the caffeine, citric acid and tartaric acid among the compounds from petroleum ether fraction and diehloromethane fraction had lithagogue effects. Both the petroleum ether fraction and dichloromethane fraction of P. lingua from Guizhou province showed good effect on prevention and treatment of calculus in middle dose groups, and the mechanism may be associated with antioxidation, reducing calcium oxalate crystal deposition, and promoting calcium oxalatecrystal release, in addition, caffeine, citric acid and tartaric acid had lithagogue effects.