共水蒸馏法提取香附-艾叶挥发油的工艺优化及与隔水蒸馏法提取的GC-MS比较

Optimization of the process of extracting essential oil from Cyperi Rhizoma-Artemisiae argyi Folium by Water distillation and comparison with Water separation distillation by GC-MS

目的研究香附-艾叶中挥发油的提取工艺,并比较共水蒸馏法与隔水蒸馏法提取药对中挥发油的差异。方法采用共水蒸馏法提取挥发油,在单因素试验的基础上,利用Box-Behnken确定香附-艾叶中挥发油的最佳提取工艺,并采用GC-MS对两种方法提取的药对及单味药材中的挥发油成分进行鉴定。结果香附-艾叶的最佳提取工艺为：提取6 h、浸泡2 h、料液比8倍;GC-MS分析表明：共水蒸馏法与隔水蒸馏法提取的挥发油中的主要成分是一致,但共水蒸馏法提取的挥发油比隔水蒸馏法的含量更高。结论所用挥发油提取工艺合理可行,可用于香附-艾叶中挥发油的提取;隔水蒸馏法提取的挥发油中的种类和含量减少;表明药对提取挥发油不是单味药的简单加合。

OBJECTIVE To study the extraction process of volatile oil from Cyperi Rhizoma-Artemisiae argyi Folium,and to compare the extraction of volatile oil from C. Rhizoma-A. argyi Folium using the water distillation and water separation distillation.METHODS The water distillation extraction was used. On the basis of single factor test,the optimum extraction process of volatile oil from C. Rhizoma-A. argyi Folium will be determined by Box-Behnken. And the volatile oil extracted using different methods will be determined by GC-MS. RESULTS The optimum extraction process for C. Rhizoma-A. argyi Folium was as follow that extraction for6 h,soaking for 2 h,material liquid ratio of 8 times. GC-MS analysis results showed that the main component of the volatile oil extracted by Water distillation and Water separation distillation were the same,but the content of volatile oil extracted by Water distillation was higher than that by Water separation distillation. CONCLUSION The proces is reasonable and feasible,and can be used for the extraction of volatile oil from C. Rhizoma-A. argyi Folium. The kinds and contents of volatile oil extracted by Water distillation were reduced. The extracted volatile oil is not the simple addition of single drug.