一种新型罗丹明B类Fe^(3+)荧光探针的合成及性能

Synthesis and Properties of Novel Rhodamine B-Based Fe^(3+) Fluorescent Probe

以罗丹明B(RhB)、水合肼、苯甲醛为原料,通过两步反应合成了一种新型罗丹明B类荧光探针RhB-P,并利用傅里叶红外变换光谱与核磁共振氢谱对RhB-P的分子结构进行表征。紫外-可见光谱和荧光光谱表明:在V(乙腈)∶V(水)=1∶1溶液中,RhB-P对Fe^(3+)具有良好的选择性,溶液可从无色转变至粉色。荧光滴定实验以及Job's实验结果表明:络合物中n(RhB-P)∶n(Fe^(3+))=1∶1,且络合物的荧光强度在c(Fe^(3+))为0.1μmol/L～20μmol/L区间范围内,与其呈正线性关系,检出限为0.193μmol/L。

A novel Rhodamine B-derived fluorescent probe（RhB-P）was synthesized through two steps with Rhodamine B,hydrazine hydrate and benzaldehyde as the raw materials. Fourier-transform infrared spectroscopyand1 H-nuclear magnetic resonance spectroscopy were utilized to investigate the chemical structure of RhB-P.UV-vis and fluorescence spectroscopy demonstrated that the probe RhB-P recognized Fe^3＋with high selectivityin the V（acetonitrile）∶V（H2 O）=1∶1 solvent,and the colorless solution turned into the pink one. Meanwhile,the fluorescence titration and Job＇s plot revealed that RhB-P combined with Fe^（3＋）into a stable chelate complex ofn（RhB-P）∶n（Fe^3＋）=1∶1,and the fluorescent intensity of the complex solution displayed a positive linearrelationship with Fe^3＋concentration in the range of 0.1 μmol/L to 20 μmol/L with 0.193 μmol/L of detection limit.