p53及CCL2在胃癌组织中的表达及临床意义

Expression and clinical significance of p53 and CCL2 in gastric cancer

目的探讨肿瘤相关蛋白53(tumor associated protein 53,p53)及CC类趋化因子配体2(CC chemokine ligand 2,CCL2)在胃癌组织中的表达水平及其与胃癌的临床病理特征之间的关系。方法应用免疫组织化学法检测191例胃癌及配对癌旁组织中p53及CCL2蛋白表达,同时将检测结果与临床病理特征进行综合分析。结果胃癌组织中p53及CCL2蛋白表达的阳性率分别为53.9%(103/191)和37.2%(71/191),而在癌旁组织中阳性率分别为14.1%(27/191)和25.1%(48/191)。胃癌组织中p53及CCL2蛋白表达阳性率均高于癌旁组织,差异均具有统计学意义(均P<0.01)。p53蛋白表达在肿瘤分化程度、浸润深度和淋巴结转移方面比较,差异均具有统计学意义(均P<0.05)。CCL2蛋白表达在肿瘤大小、浸润深度和TNM临床分期方面比较,差异均具有统计学意义(均P<0.05)。结论 p53及CCL2蛋白表达情况与胃癌组织生长关系密切,其表达情况可能成为胃癌恶性程度的一个良好指标。

Objective To investigate the expression of tumor associated protein 53（ p53） and CC chemokine ligand2（ CCL2） in gastric cancer,and to analyze their relationship with the clinical characteristics of gastric cancer. Methods Immunohistochemistry was used to detect the expression of p53 and CCL2 in 191 samples of gastric cancer and matched adjacent tissues,and the correlation of p53 and CCL2 expression with the clinicopathological characteristics of the patients was analyzed. Results Among 191 pairs of specimens,the positive expression rates of p53 and CCL2 protein in gastric cancer tissues were 53. 9%（ 103/191） and 37. 2%（ 71/191）,respectively,whereas those in pericancerous tissues were 14. 1%（ 27/191） and 25. 1%（ 48/191）,respectively（ both P 0. 01）. The expression of p53 protein was significantly different in terms of tumor differentiation,depth of invasion,and lymph node metastasis（ all P 0. 05）. The expression of CCL2 was significantly different in terms of tumor size,depth of invasion,and TNM stage（ all P 0. 05）. Conclusion p53 and CCL2 expression levels are closely correlated with the development of gastric tumor,which can be used as biomarkers for the tumor malignancy of gastric cancer.