灵芝蛋白质的优化提取及其抗氧化性质研究

Optimization of Extraction and Studies on Antioxidant Properties of Ganoderma lucidum Protein

采用正交试验法优化灵芝蛋白质提取工艺,并对其抗氧化活性进行表征。以灵芝蛋白质的提取率为指标,考察料液比(m/V)、时间、温度及pH对提取率的影响。在单因素试验的基础上,采用L9(34)正交设计方法确定灵芝蛋白质的最优提取工艺。结果表明:灵芝蛋白质最佳提取工艺条件为:料液比(m/V)1∶120、温度65℃、p H 10.0,提取率达50.52%。在最优条件下提取的灵芝蛋白对DPPH自由基和羟基自由基的半数清除质量浓度分别为685.02μg/m L和568.82μg/m L,对ABTS自由基的清除率和金属螯合率在200μg/m L时分别达到99.60%和83.15%,具有较强的还原力。由此说明灵芝蛋白质具有较高的抗氧化活性。

The optimization of the extraction process of protein from Ganoderma lucidum was achieved by orthogonal experiment method and its antioxidant activity were characterized. Ganoderma lucidum protein extraction rate was taken as the index to study the effect of ratio of material： liquid（m/V）, extraction time, temperature and p H on extraction yield.On the basis of single factor experiment, using L9（34） orthogonal design and analysis method was taken to determine the Ganoderma lucidum protein extraction process. The results showed that a material： liquid（m/V） of 1 ∶120, a extraction temperature of 65 ℃, a p H of 10.0 a p H were optimal for protein extraction from Ganoderma lucidum and the extraction ratio was 50.52%. The concentration for 50% of maximal effect（EC50） of Ganoderma lucidum protein extracted under the optimal condition to DPPH free radicals and hydroxyl radicals were 68 502 μg/m L and 568.82 μg/m L, ABTS free radical scavenging rate and metal chelating rate in the 200 μg/m L of 99.60% and 83.15% respectively, and has stronger reducing power, which showed that protein extraction from Ganoderma lucidum has certain antioxidant activity.