不同粒径的石墨烯量子点在PC12细胞中的成像效应及细胞毒性

Biological imaging effect and biocompatibility of graphene quantum dots(GQDs) of different size in PC12 cells

目的探究不同粒径的石墨烯量子点(graphene quantum dots,GQDs)在PC12细胞中的细胞成像效应及细胞毒性。方法通过动态光散射法(DLS)对GQDs的水合粒径和zeta电位进行表征,采用CCK-8试剂盒检测GQDs对PC12细胞的毒理效应,使用激光共聚焦显微镜比较不同粒径的GQDs在PC12细胞中的荧光成像效应。结果 GQDs对PC12细胞的毒性效应是呈尺寸依赖性的,15 nm的GQDs比50 nm GQDs对PC12细胞的细胞毒性低,500μg/ml的15 nm GQDs孵育48 h后,细胞活力仍保持在80%以上。对15 nm的GQDs更容易被PC12细胞摄取,80%以上的细胞能成功显影,表现优异的细胞成像效应。结论 GQDs细胞毒性低,细胞成像效果好,是一种适合神经系统成像的纳米材料。

Objective To evaluate the biological imaging effect and biocompatibility of graphene quantum dots（ GQDs） of different size in PC12 cells. Methods The hydrodynamic size and zeta potencial of GQDs in distilled water（ DW） were determined by dynamic light scattering（ DLS）. Furthermore,cell survival rates following treatment with GQDs were evaluated using the CCK-8 assay and the biological imaging effect of GQDs in PC12 cells was observed under a confocal microscope. Results The toxic effect of GQDs in PC12 cells was size-dependent. The results of CCK-8 assay indicated that 15 nm GQDs showed lower toxicity than CDs in PC12 cells. After PC12 cells were incubated 48 h with 500 μg/ml GQDs,cell viability remained at 80% or more. What＇s more,15 nm GQDs could be taken up by PC12 cells more easily and more than 80% cells could be labeled successfully,which indicated good biological imaging effects. Conclusions GQDs have low cytotoxicity and excellent biological imaging properties,which are a suitable nanomaterial for neuroimaging.