甲基转移酶3在乳腺癌组织的表达及其临床意义

Expression of methyltransferase 3 in breast cancer and clinical significance

目的观察甲基转移酶3（METYL3）在乳腺癌组织中的表达，分析其与乳腺癌临床病理特征的关系，探讨METY[3对乳腺癌细胞发生发展的影响。方法随机收集54例乳腺癌患者的癌组织和癌旁正常组织，Westernblot检测比较METYL3在乳腺癌及癌旁组织中的表达水平，实时荧光定量聚合酶链反应（FQ-PCR）方法检测不同病理特征的乳腺癌和癌旁组织中METrL3mRNA的表达水平，分析其表达与乳腺癌临床病理特征的关系。结果ME3TL3mRNA在乳腺癌组织中的表达明显高于癌旁组织，差异有统计学意义（P=0．001），METYL3在乳腺癌组织中的表达与患者年龄、肿瘤大小、雌激素受体（ER）及孕激素受体（PR）状态无明显相关（χ2=1．856，P=0．173；χ2=0．351，P=0．554;χ2=0．037，P=0．847；χ2=2．598，P=0．107），而与乳腺癌腋窝淋巴结转移、细胞核增殖抗原（Ki-67）、人表皮生长因子受体2（Her-2）的表达明显相关（χ2=4．913，P=0．027；χ2=7．517，P=0．006；χ2=10．165，P=0．001）。Westernblot检测显示METTL3在乳腺癌组织的表达水平（4．691±0．373）明显高于癌旁组织（2．238±0．362），差异有统计学意义（P=0．016）。结论METTL3在乳腺癌组织中的表达高于癌旁正常组织，且与乳腺癌腋窝淋巴结转移、Ki-67、Her-2的表达密切相关，表明METFL3在乳腺癌细胞增殖和侵袭过程中发挥重要作用。

Objective To study the expression level of methyltransferase 3 （METTL3） in the breast cancer, analyze the correlation between METFL3 and clinicopathological characteristics of breast cancer, and explore possible mechanisms of the involvement of METYL3 in the occurrence and development of breast cancer. Methods 54 cases of breast carcinoma and para - carcinoma tissues were collected. Western blotting was used to detect the protein expression of METFL3 in breast carcinoma and adjacent tissues. Real - time fluorescent quantitative polymerase chain reaction （ FQ - PCR） method was operated to detect the expression of METTL3 mRNA and the correlation between METYL3 and elinieopathological characteristics of breast cancer. Results The expression of METrL3 in breast cancer was significantly higher than in the adjacent tissues （ P = 0. 001 ）. The expression of METFL3 mRNA was not related to age, the size of tumor, status of estrogen receptor （ ER）, and progesterone receptor （PR） （χ2 = 1. 856, P = χ2 0. 173; =0.351, P=0.554;χ2 =0.037, P=0. 847;χ2 =2.598, P=0. 107）, but to lymph node me- tastasis, human epidermal growth factor receptor -2 （Her -2 ） and proliferation cell nuclear antigen （ Ki - 67 ） （χ2 = 4. 913, P = 0. 027 ; χ2 = 7. 517, P = 0. 006 ; χ2 = 10. 165, P = 0. 001 ）, Western blotting showed that the expression of METFL3 in breast cancer （4. 691 ± 0. 373 ） was higher than in the normal tissue （2. 238 ± 0. 362） （P = 0. 016）. Conclusion The expression of METI＇L3 in breast cancer is higherthan in normal tissues, and is correlated with lymph node metastasis, depth of invasion and TNM staging, suggesting their roles in the breast cancer.