西藏大麦黄矮病病毒株系鉴定及介体蚜虫传毒能力分析

Identification of Strain of Tibet Barley Yellow Dwarf Virus and Analysis of Transmission Capabilities of Mediating Aphid

从西藏青稞发病田采集黄矮病株10份,经生物学分离并用BYDV-GAV、PAV、RPV、SGV 4种抗血清进行酶联免疫吸附(即EILSA法)测定和RT-PCR法验证。拉萨曲水6份标样与GAV抗血清有强反应,而与PAV、RPV、SGV抗血清无反应,说明拉萨曲水大麦黄矮病毒的主要株系为GAV株系。采用无毒麦无网长管蚜、麦长管蚜、禾谷缢管蚜传毒,麦长管蚜对GAV株系病毒传毒强,拉萨、山南两地的麦长管蚜传毒力无明显差异。

Ten barley with yellow dwarf diseases were collected from the infected field of Tibetan highland barley, and were isolated biologically and enzyme-linked immunosorbent assay （EILSA） with BYDV-GAV, PAV, RPV and SGV. Moreover, RT-PCR method was used for verification. Six standard samples of Lhasa Qushui have a strong reaction with GAV antiserum. However, they have no reaction with PAV,RPV and SGV antiserum. This means that the main strain of Lhasa Qushui barley yellow dwarf virus is GAV strain, which uses the non-toxic Metopolophium dirhodum （Walker）, Sitobion avenae （Fabricius） and Rhopalosiphum padi （Linnaeus） to transmit virus. Moreover, Sito-bion avenae （Fabricius） has the strongest ability of the virus transmission for GAV strains, and the ability of the virus transmission of Sito-bion avenae （Fabricius） in Lhasa and Shannan has no obvious difference.