Toll样受体2在结核分枝杆菌诱导中性粒细胞凋亡中的作用

The role of Toll-like receptor 2 in the apoptosis of neutrophils induced by mycobacterium tuberculosis

目的探讨Toll样受体2(TLR2)在结核分枝杆菌(M. tb)诱导的中性粒细胞(PMN)凋亡中的作用。方法用M.tb感染人外周血分离的PMN,采用流式细胞仪检测细胞凋亡率及细胞表面TLR2表达情况,并观察阻断TLR2后PMN感染M.tb的凋亡变化。结果 M. tb感染PMN后细胞凋亡率随时间延长逐渐增加,6h后凋亡率明显高于对照组,差异具有统计学意义(P <0. 05)。阻断TLR2可以明显降低感染M. tb后的PMN凋亡率。结论 TLR2在M. tb诱导PMN凋亡中起重要作用。

Objective To study the roll of Toll-like receptor 2（ TLR2） in the apoptosis of neutrophils（ PMN） induced by mycobacterium tuberculosis（ M. tb）. Methods The fresh PMNs were isolated from peripheral blood of healthy people and infected with M. tb. The apoptosis rate of PMN and the expression of TLR2 in PMN membrane was detected by flow cytometry（ FCM）. With pretreatment of the TLR2 monoclonal antibody,the apoptosis rate of PMNs infected with M. tb was detected by flow cytometry. Results With infection of M. tb,the apoptosis rare of PMN was increased. The apoptosis rate of was significantly higher in M. tb induced group than that in normal control group from 6 to 24 h,and the difference was statistically significant（ P 〈 0. 05）. Blocking TLR2 could decreased the apoptosis rate of PMN infected with M. tb. Conclusion TLR2 was associated with the M. tb-induced apoptosis of PMN.