蓖麻RcDella(GAI)基因克隆及生物信息学分析

Cloning and Bioinformatics Analysis of RcDella(GAI) Gene in Castor

DELLA蛋白是GRAS蛋白家族中的亚家族,在赤霉素信号转导通路中起负调控作用。本研究通过对DELLA蛋白编码基因的克隆、表达情况,及对RcDella (GAI)基因进行生物信息学分析表明,此基因片段包含1 704 bp组成的开放阅读框(ORF),编码567个氨基酸,分子量为62 550.40 Da,pI=5.14,为亲水性不稳定蛋白;发现RcDella (GAI)蛋白隶属GRAS Superfamily家族和DELLA Superfamily家族,为非分泌蛋白,有58个磷酸化位点。同源性分析结果表明,与大戟科麻风树、木薯和橡胶树高度同源。DELLA蛋白在大肠杆菌中以可溶蛋白表达,质谱分析显示分子量为62.5 kD,确定为RcDella (GAI)。这些分析结果为进一步研究DELLA蛋白在蓖麻生长发育过程中功能作用提供了理论依据。

DELLA protein is a subfamily in the GRAS protein family, and it plays a negative regulatory role in the gibberellin signal transduction pathway. In this study, we cloned and expressed the gene encoding DELLA protein, and analyzed the bioinformatics ofRcDella （GA I） gene. The results showed that this gene fragment contained an o- pen reading frame （ORF） of 1 704 bp, encoding 567 proteins, with the molecular weight of 62 550.40 Da and pI of 5.14, which was a hydrophilic unstable protein. It was also found that RcDella （GAD protein belonged to the GRAS Superfamily family and DELLA Superfamily family, which was a non-secretory protein with 58 phosphorylation sites. Homology analysis indicated that it had high homology with Euphorbiaceae Jatropha, cassava, and rubber trees. These results laid the foundation for the further study on the functional role of DELLA protein in the growth and development of castor.