电针刺激对大鼠脊髓损伤后炎症反应的影响

Effects of Electroacupuncture on Inflammatory Response in Spinal Cord Injury of Rats

目的:观察不同配穴电针方式对脊髓损伤(SCI)大鼠IL-lβ,IL-6及IL-10表达的影响,探讨电针在脊髓损伤修复过程中的作用机制。方法:48只SD大鼠随机分为4组:假手术组(12例)、对照组(12例)、夹脊电针组(12例)、督脉电针组(12例)。术后第7天对各组大鼠进行BBB运动功能评分,并采用苏木精-伊红及Nissle染色观察组织细胞形态变化,采用实时荧光定量PCR法检测白介素-lβ(ILlβ),白介素-6(IL-6)和白介素-10(IL-10)mRNA的表达情况。结果:术后第7天对照组、夹脊电针组、督脉电针组BBB评分均低于假手术组(P<0.05),夹脊电针组、督脉电针组BBB评分均高于对照组(P<0.05);对照组、夹脊电针组、督脉电针组IL-lβ,IL-6及IL-10mRNA的表达含量均高于假手术组(P<0.05);夹脊电针组、督脉电针组IL-lβ,IL-6mRNA的表达含量均低于对照组(P<0.05);夹脊电针组、督脉电针组IL-10mRNA的表达含量均高于对照组(P<0.05);夹脊电针组与督脉电针组IL-lβ,IL-6及IL-10mRNA的表达差异无统计学意义(P>0.05)。结论:夹脊、督脉电针均可以改善大鼠SCI后下肢运动功能,降低IL-lβ和IL-6的表达,增加IL-10的表达,抑制SCI后炎症反应。

Objective：To observe the effect of electroacupuncture treatment（EA）at different acupoints on the expression of IL-1β,IL-6 and IL-10 in rats with spinal cord injury（SCI）and discuss the role and mechanism of electroacupuncture in the repair process of spinal cord injury.Methods：A total of 48 SD rats were randomly divided into 4 groups：sham operation group（n=12）,control group（n=12）,Jiaji EA group（n=12）,and Du Meridian EA group（n=12）.Scores of Basso Beattie Bresnahan（BBB）motor functions were assessed at 7 th days after operation.Histopathological changes were observed by HE and Nissle staining.The expressions of IL-1β,IL-6 and IL-10 mRNA were detected by quantitative real time-PCR（qRT-PCR）.Results：The BBB scores of the control group,Jiaji EA group,and Du meridians EA group were lower than the sham operation group on the 7 th day after operation（P〈0.05）.The BBB scores of Jiaji EA group and Du meridians EA group were higher than the control group（P〈0.05）.The expression levels of IL-1β,IL-6 and IL-10 mRNA in the control group,Jiaji EA group and Du meridians EA group were higher than those in the sham operation group（P〈0.05）.The expression levels of IL-1βand IL-6 mRNA in the Jiaji EA group and Du meridian EA group were lower than those in the control group（P〈0.05）.The expression levels of IL-10 mRNA in Jiaji EA group and Du meridians EA group were high than those in the control group（P〈0.05）.The expressions of IL-1β,IL-6 and IL-10 mRNA in Jiaji EA group and Du meridian EA group had no statistical significance（P〉0.05）.Conclusion：Jiaji and Du Meridian EA could improve the motor function of the SD rats’ lower limb,reduce the expression of IL-1βand IL-6 and increase expression of IL-10 and inhibit the inflammatory response after SCI.