间质-上皮转化参与子宫内膜异位症模型大鼠包囊形成

Mesenchymal epithelial transformation is involved in the formation of cysts in endometriosis rats

目的探讨间质上皮转化以及转录因子Snail在子宫内膜异位症(EMS)模型大鼠异位包囊形成中的作用。方法自体移植法建立EMS大鼠模型。选取造模成功形成包囊的EMS大鼠,分为造模后2周至7周组,取正常大鼠为正常对照组。采用免疫组织化学方法研究上皮标志物(E-钙黏蛋白、角蛋白8)、间质标志物(波形蛋白)以及Snail在异位内膜包囊中的定位和表达情况,Western blotting法检测E-钙黏蛋白、波形蛋白在异位内膜包囊形成组织中的含量。采用TUNEL法检测异位包囊内部脱落细胞凋亡情况。结果免疫组织化学结果显示,EMS模型大鼠的异位内膜包囊腔上皮E-钙黏蛋白、角蛋白8和波形蛋白含量在造模后2~7周过程中,相对于包囊壁间质层均处于较高水平,而且与正常子宫内膜上皮比较,亦处于较高的水平。另发现造模后2~7周异位包囊腔上皮样细胞脱落,E-钙黏蛋白、角蛋白8、波形蛋白阳性表达,且在造模后4~5周阳性信号最强。与正常大鼠子宫内膜组织比较,造模后3~5周异位内膜包囊腔上皮中Snail阳性表达信号增强。Western blotting法检测显示,造模后2~7周异位内膜中E-钙黏蛋白、波形蛋白含量与正常内膜比较,均维持在较高水平,差异均有统计学意义(P<0.05)。TUNEL法检测发现异位包囊内部脱落细胞未发生凋亡。结论 EMS模型大鼠异位包囊腔上皮细胞可能是由间质细胞转化形成,提示间质-上皮转化(MET)参与了异位包囊形成,且Snail参与调控其过程;异位内膜包囊上皮细胞脱落以形成囊腔,但其上皮细胞脱落不伴随细胞凋亡。

Objective To investigate the role of stromal epithelial transformation and Snail in ectopic cyst formation in endometriosis（EMS） rats. Methods The model of EMS rats was established by autologous transplantation. EMS rats were successfully divided into 2-7 weeks after modeling. The normal rats were taken as the normal control group. Immunohistochemical method was used to study the localization and expression of epithelial marker（E-cadherin, cytokeratin 8）, mesenchymal marker（vimentin） and Snail in ectopic endometrial