原癌基因c-Ski在非小细胞肺癌组织中的表达及与肿瘤早期转移的关系

Relationship between the expression of proto oncogene c - Ski in non - small cell lung cancer and the early metastasis of tumor

目的 研究原癌基因c-Ski在非小细胞肺癌组织中的表达及与早期转移的临床关系.方法 选取2015年1月至2016年7月在萍乡市人民医院治疗的非小细胞肺癌患者97例.应用免疫组化及Real-Time PCR法分别检测肺癌组织及癌旁组织中c-Ski蛋白及mRNA表达情况.分析c-Ski表达与患者临床病理表现之间的关系并讨论其与肿瘤发生早期转移的关系.结果 免疫组化分析显示癌旁组织及癌组织中c-Ski蛋白的表达分别为18.6％及79.4％,两者差异有统计学意义（χ^2=71.80,P〈0.01）;c-Ski蛋白的表达与年龄、分期及肿瘤细胞来源相关,差异均有统计学意义（χ^2=8.40、4.72、15.71,均P〈0.05）.结论 原癌基因c-Ski表达可能在非小细胞肺癌侵袭和转移潜能中起重要作用,可成为非小细胞肺癌的一个潜在的治疗靶点.

Objective To explore the relationship between the expression of proto oncogene c -Ski in non-small cell lung cancer and the early metastasis of tumor .Methods From January 2015 to July 2016 , 97 patients with non -small cell lung cancer in the People＇s Hospital of Pingxiang were selected .The protein expression of c-Ski was examined with immunohistochemical SP and real -time PCR,the results were observed and compared.Results The positive rates of c-Ski protein and mRNA level in non -small cell lung cancer tissues and adjacent normal tissues were 18.6%and 79.4%,respectively,the difference was statistically significant （χ^2 =71.80, P〈0.01）.The positive rate of c-Ski protein expressed in non -small cell lung cancer patients was related with age , clinical stage and pathologic classification ,the differences were statistically significant （χ^2 =8.40,4.72,15.71,all P〈0.05）.Conclusion c-Ski may play an important role in the invasion and metastasis of non -small cell lung cancer,which can be used as a potential therapeutic target for non -small cell lung cancer in the future .