扇贝抗氧化肽对衰老小鼠体内抗氧化活性研究

Antioxidant function of peptides from scallop by-product in aged mice in vivo

目的研究扇贝副产物抗氧化肽对衰老小鼠体内抗氧化活性。方法采用D-半乳糖构建小鼠衰老动物模型,分别以不同性别小鼠肝脏中特征酶超氧化物歧化酶(superoxide dismutase,SOD),过氧化氢酶(catalase, CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活性及丙二醛(alondialdehyde, MDA)含量为指标,评价扇贝抗氧化肽的体内抗氧化功能。结果与模型组比较,灌胃剂量为0.5 mL/kg的样品组能够显著提高模型小鼠(雌性和雄性)中肝脏中SOD、CAT、GSH-Px等特征酶的活性,并降低MDA含量水平。同时以脾脏指数、胸腺指数、吞噬指数为指标评价扇贝抗氧化肽对小鼠免疫能力的影响。结果显示灌胃剂量为0.50mL/kg和1.0mL/kg的扇贝抗氧化肽能够提高小鼠的脾脏指数、胸腺指数和吞噬指数。结论扇贝抗氧化肽具有良好的增强机体抗氧化功能作用。

Objective To investigate the antioxidant function of peptides from scallop by-product in aged mice in vivo. Methods D-galactose was used to construct an animal model of aged mice. Superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GSH-px） and the content of alondialdehyde（MDA） in the liver of mice of different genders were used as indicators to evaluate the antioxidant function of the anti-oxidant peptide in scallop. Results Compared with the model group, the intragastric dosage of 0.5 mL/kg in the sample group was able to significantly increase the activity of SOD, CAT, gsh-px and other characteristic enzymes in the liver of the model mice（female and male） and reduce the MDA level. At the same time, spleen index, thymus index and phagocytic index were used to evaluate the effect of scallop anti-oxidant peptide on mice immunity. The results showed that the intragastric dosage of 0.50 mL/kg and 1.0 mL/kg of scallop anti-oxidant peptide could improve the spleen index, thymus index and phagocytic index of mice. Conclusion The anti-oxidant peptide of scallops has a good effect on enhancing the antioxidant function of the body.